ArticleInvestigative ophthalmology & visual science2014
Scleral permeability varies by mouse strain and is decreased by chronic experimental glaucoma.
Article in Investigative ophthalmology & visual science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 20 citations in OpenAlex.
- Comparison of the Biomechanics of the Mouse Astrocytic Lamina Cribrosa Between Glaucoma and Optic Nerve Crush Models.Investigative ophthalmology & visual science · 2023Article
- Aging Effects on Optic Nerve Neurodegeneration.International journal of molecular sciences · 2023Review
- CXC- receptor 2 promotes extracellular matrix production and attenuates migration in peripapillary human scleral fibroblasts under mechanical strain.Journal of cellular and molecular medicine · 2022Article
- Neuromechanobiology: An Expanding Field Driven by the Force of Greater Focus.Advanced healthcare materials · 2021Review
- Collagen Structural Changes in Rat Tarsus After Crosslinking.Translational vision science & technology · 2021Article
- Biomechanics of the optic nerve head and peripapillary sclera in a mouse model of glaucoma.Journal of the Royal Society, Interface · 2020Article
- Changes in Collagen Structure and Permeability of Rat and Human Sclera After Crosslinking.Translational vision science & technology · 2020Article
- The Dynamic Scleral Extracellular Matrix Alterations in Chronic Ocular Hypertension Model of Rats.Frontiers in physiology · 2020Article
- Controlled release of corticosteroid with biodegradable nanoparticles for treating experimental autoimmune uveitis.Journal of controlled release : official journal of the Controlled Release Society · 2019Article
- Unconventional aqueous humor outflow: A review.Experimental eye research · 2017Review
- Measuring Deformation in the Mouse Optic Nerve Head and Peripapillary Sclera.Investigative ophthalmology & visual science · 2017Article
- Scleral fibroblast response to experimental glaucoma in mice.Molecular vision · 2016Article
- Optic nerve head biomechanics in aging and disease.Experimental eye research · 2015Review
- Translating ocular biomechanics into clinical practice: current state and future prospects.Current eye research · 2015Review
- Losartan Treatment Protects Retinal Ganglion Cells and Alters Scleral Remodeling in Experimental Glaucoma.PloS one · 2015Article
- Experimental scleral cross-linking increases glaucoma damage in a mouse model.Experimental eye research · 2014Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
purposeTo determine differences in scleral permeability, as measured by diffusion of macromolecules, by using fluorescence recovery after photobleaching (FRAP), with reference to differences by mouse strain, scleral region, and the effect of experimental glaucoma.
methodsIn three mouse strains (B6, CD1, and B6 mice with mutation in collagen 8α2 [Aca23]), we used FRAP to measure the diffusion of fluorescein isothiocyanate-dextran, molecular weight 40 kDa, into a photobleached zone of sclera. Scleral regions near the optic nerve head (peripapillary) and two successively more anterior regions were compared. Sclera from mouse eyes subjected to chronically elevated intraocular pressure after bead injection into the anterior chamber were compared to fellow eye controls. FRAP data were compared against estimated retinal ganglion cell axon loss in glaucomatous eyes.
resultsDiffusion rates of dextran molecules in the sclera were significantly greater in Aca23 and B6 mice than in CD1 mice in a multivariate model adjusted for region and axial length (P < 0.0001). Dextran diffusion significantly decreased in glaucomatous eyes, and the decline increased with greater axon loss (P = 0.0003, multivariable model). Peripapillary scleral permeability was higher in CD1 than B6 and Aca23 mice (P < 0.05, multivariable model, adjusted by Bonferroni).
conclusionsMeasurement of the diffusion rates of dextran molecules in the sclera showed that glaucoma leads to decreased scleral permeability in all three mouse strains tested. Among mouse strains tested, those that were more susceptible to glaucomatous loss of retinal ganglion cells had a lower scleral permeability at baseline.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.