ArticleNature chemistry2014
A protein engineered to bind uranyl selectively and with femtomolar affinity.
Article in Nature chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed.
- Chemical Synthesis of Functionalized Peptidomelanin for Groundwater Uranium Extraction.ACS omega · 2026Article
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- Conditional Protein Structure Generation with Protpardelle-1c.bioRxiv : the preprint server for biology · 2025Article
- Protein with twin binding sites for uranium extraction from seawater.National science review · 2025Article
- A low-toxicity uranyl-selective-binding linear pentapeptide sequence as a potential uranium decorporation agent.RSC advances · 2024Article
- An imidazole-based covalent-organic framework enabling a super-efficiency in sunlight-driven uranium extraction from seawater.Chemical science · 2024Article
- Synthetic and Computational Design Insights toward Mimicking Protein Binding of Phosphate.Bioconjugate chemistry · 2024Article
- DRGD-linked charged EKKE dimeric dodecapeptide: pH-based amyloid nanostructures and their application in lead and uranium binding.RSC advances · 2024Article
- Solar-powered simultaneous highly efficient seawater desalination and highly specific target extraction with smart DNA hydrogels.Science advances · 2023Article
- Characterization of Uranyl (UOACS chemical neuroscience · 2023Article
- Atomically Accurate Design of Metalloproteins with Predefined Coordination Geometries.Journal of the American Chemical Society · 2023Article
- Article
- Directed assembly of genetically engineered eukaryotic cells into living functional materials via ultrahigh-affinity protein interactions.Science advances · 2022Article
- Article
- Highly Efficient Electrocatalytic Uranium Extraction from Seawater over an Amidoxime-Functionalized In-N-C Catalyst.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Multi-scale computer-aided design and photo-controlled macromolecular synthesis boosting uranium harvesting from seawater.Nature communications · 2022Article
- Article
- Protein-protein interaction prediction with deep learning: A comprehensive review.Computational and structural biotechnology journal · 2022Review
- De novo metalloprotein design.Nature reviews. Chemistry · 2022Article
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11 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Uranyl (UO2(2+)), the predominant aerobic form of uranium, is present in the ocean at a concentration of ~3.2 parts per 10(9) (13.7 nM); however, the successful enrichment of uranyl from this vast resource has been limited by the high concentrations of metal ions of similar size and charge, which makes it difficult to design a binding motif that is selective for uranyl. Here we report the design and rational development of a uranyl-binding protein using a computational screening process in the initial search for potential uranyl-binding sites. The engineered protein is thermally stable and offers very high affinity and selectivity for uranyl with a Kd of 7.4 femtomolar (fM) and >10,000-fold selectivity over other metal ions. We also demonstrated that the uranyl-binding protein can repeatedly sequester 30-60% of the uranyl in synthetic sea water. The chemical strategy employed here may be applied to engineer other selective metal-binding proteins for biotechnology and remediation applications.
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