ArticleMolecular & cellular proteomics : MCP2014
An unbiased proteomic screen reveals caspase cleavage is positively and negatively regulated by substrate phosphorylation.
Article in Molecular & cellular proteomics : MCP, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
- Caspase-mediated cleavage events hidden by secondary proteolysis during apoptosis.Protein science : a publication of the Protein Society · 2026Article
- Phosphorylation shapes antigen presentation and immune recognition: mechanistic insights and translational perspectives.Frontiers in immunology · 2026Review
- Apoptotic Caspases-3 and -7 Cleave Extracellular Domains of Membrane-Bound Proteins from MDA-MB-231 Breast Cancer Cells.International journal of molecular sciences · 2025Article
- An atlas of caspase cleavage events in differentiating muscle cells.Protein science : a publication of the Protein Society · 2024Article
- Comparison of CX-4945 and SGC-CK2-1 as inhibitors of CSNK2 using quantitative phosphoproteomics: Triple SILAC in combination with inhibitor-resistant CSNK2.Current research in chemical biology · 2023Article
- Microfluidic deep mutational scanning of the human executioner caspases reveals differences in structure and regulation.Cell death discovery · 2022Article
- Chemical Genetic Validation of CSNK2 Substrates Using an Inhibitor-Resistant Mutant in Combination with Triple SILAC Quantitative Phosphoproteomics.Frontiers in molecular biosciences · 2022Article
- Non-Apoptotic Caspase Activity Preferentially Targets a Novel Consensus Sequence Associated With Cytoskeletal Proteins in the Developing Auditory Brainstem.Frontiers in cell and developmental biology · 2022Article
- Species-Specific Endotoxin Stimulus Determines Toll-Like Receptor 4- and Caspase 11-Mediated Pathway Activation Characteristics.mSystems · 2021Article
- Evaluation of the effects of phosphorylation of synthetic peptide substrates on their cleavage by caspase-3 and -7.The Biochemical journal · 2021Article
- Posttranslational and Therapeutic Control of Gasdermin-Mediated Pyroptosis and Inflammation.Frontiers in immunology · 2021Review
- Caspase-3 Cleaves Extracellular Vesicle Proteins During Auditory Brainstem Development.Frontiers in cellular neuroscience · 2020Article
- Phosphorylation-mediated activation of mouse Xkr8 scramblase for phosphatidylserine exposure.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- TAILS N-terminomics and proteomics reveal complex regulation of proteolytic cleavage byThe Journal of biological chemistry · 2018Article
- Evolution of caspase-mediated cell death and differentiation: twins separated at birth.Cell death and differentiation · 2017Review
- Metacaspases versus caspases in development and cell fate regulation.Cell death and differentiation · 2017Review
- Cacidases: caspases can cleave after aspartate, glutamate and phosphoserine residues.Cell death and differentiation · 2016Article
- A bacterial type III secretion-based protein delivery tool for broad applications in cell biology.The Journal of cell biology · 2015Article
- Caspase 3 cleavage of Pax7 inhibits self-renewal of satellite cells.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Enterococcus faecalis Gelatinase Mediates Intestinal Permeability via Protease-Activated Receptor 2.Infection and immunity · 2015Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Post-translational modifications of proteins regulate diverse cellular functions, with mounting evidence suggesting that hierarchical cross-talk between distinct modifications may fine-tune cellular responses. For example, in apoptosis, caspases promote cell death via cleavage of key structural and enzymatic proteins that in some instances is inhibited by phosphorylation near the scissile bond. In this study, we systematically investigated how protein phosphorylation affects susceptibility to caspase cleavage using an N-terminomic strategy, namely, a modified terminal amino isotopic labeling of substrates (TAILS) workflow, to identify proteins for which caspase-catalyzed cleavage is modulated by phosphatase treatment. We validated the effects of phosphorylation on three of the identified proteins and found that Yap1 and Golgin-160 exhibit decreased cleavage when phosphorylated, whereas cleavage of MST3 was promoted by phosphorylation. Furthermore, using synthetic peptides we systematically examined the influence of phosphoserine throughout the entirety of caspase-3, -7, and -8 recognition motifs and observed a general inhibitory effect of phosphorylation even at residues considered outside the classical consensus motif. Overall, our work demonstrates a role for phosphorylation in controlling caspase-mediated cleavage and shows that N-terminomic strategies can be tailored to study cross-talk between phosphorylation and proteolysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.