ArticleNature protocols2014
Preparation of the planarian Schmidtea mediterranea for high-resolution histology and transmission electron microscopy.
Article in Nature protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 35 citations in OpenAlex.
- A Field-Friendly, Non-Toxic Fixative for Integrated Morphological and Molecular Research in Non-Model Invertebrates.Ecology and evolution · 2026Article
- Enhanced RNA quality control maintains long-term regenerative ability in planarians.Development (Cambridge, England) · 2025Article
- Mitochondrial dynamics govern whole-body regeneration through stem cell pluripotency and mitonuclear balance.Nature communications · 2024Article
- Quantitative Analysis of Planarian Pigmentation.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Functional optical coherence tomography enablesBiomedical optics express · 2020Article
- A planarian nidovirus expands the limits of RNA genome size.PLoS pathogens · 2018Article
- Light-Induced Length Shrinkage of Rod Photoreceptor Outer Segments.Translational vision science & technology · 2018Article
- Article
- Identification of Autophagy-Related Gene 7 and Autophagic Cell Death in the PlanarianFrontiers in physiology · 2018Article
- Cytoplasmic poly (A)-binding protein critically regulates epidermal maintenance and turnover in the planarianDevelopment (Cambridge, England) · 2017Article
- A functional genomics screen in planarians reveals regulators of whole-brain regeneration.eLife · 2016Article
- Mass Spectrometry Imaging and Identification of Peptides Associated with Cephalic Ganglia Regeneration in Schmidtea mediterranea.The Journal of biological chemistry · 2016Article
- Forkhead containing transcription factor Albino controls tetrapyrrole-based body pigmentation in planarian.Cell discovery · 2016Article
- Tryptophan hydroxylase Is Required for Eye Melanogenesis in the Planarian Schmidtea mediterranea.PloS one · 2015Article
- Generation of cell type-specific monoclonal antibodies for the planarian and optimization of sample processing for immunolabeling.BMC developmental biology · 2014Article
- A Practical Approach to Study Uncultivated Protists Using Single-Cell Techniques for Electron Microscopy.The Journal of eukaryotic microbiologyArticle
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
Abstract
The flatworm Schmidtea mediterranea is an emerging model species in fields such as stem cell biology, regeneration and evolutionary biology. Excellent molecular tools have been developed for S. mediterranea, but ultrastructural techniques have received far less attention. Processing specimens for histology and transmission electron microscopy (TEM) is notoriously idiosyncratic for particular species or specimen types. Unfortunately, however, most methods for S. mediterranea described in the literature lack numerous essential details, and those few that do provide them rely on specialized equipment that may not be readily available. Here we present an optimized protocol for ultrastructural preparation of S. mediterranea. The protocol can be completed in 6 d, much of which is 'hands-off' time. To aid with troubleshooting, we also illustrate the major effects of seemingly minor variations in fixative, buffer concentration and dehydration steps. This procedure will be useful for all planarian researchers, particularly those with relatively little experience in tissue processing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.