ArticleMethods (San Diego, Calif.)2014
Targeted genome editing in Aedes aegypti using TALENs.
Article in Methods (San Diego, Calif.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 41 citations in OpenAlex.
- Split suppression gene drives reveal a design tradeoff between inheritance and reproductive load inbioRxiv : the preprint server for biology · 2026Article
- Arthropod promoters for genetic control of disease vectors.Trends in parasitology · 2024Review
- Transgene removal using an in cis programmed homing endonuclease via single-strand annealing in the mosquito Aedes aegypti.Communications biology · 2024Article
- CRISPR-Cas9 Genome Editing Uncovers the Mode of Action of Methoprene in the Yellow Fever Mosquito,The CRISPR journal · 2022Article
- Article
- Functional Analysis of Individual piRNAs in Aedes aegypti Cells and Embryos Using Antisense Oligonucleotides.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Expanding the Toolkit for Genome Editing in a Disease Vector,The CRISPR journal · 2021Article
- Novel synthetic 3'-untranslated regions for controlling transgene expression in transgenicRNA biology · 2021Article
- A piRNA-lncRNA regulatory network initiates responder and trailer piRNA formation during mosquito embryonic development.RNA (New York, N.Y.) · 2021Article
- A selectable, plasmid-based system to generate CRISPR/Cas9 gene edited and knock-in mosquito cell lines.Scientific reports · 2021Article
- CRISPR/Cas9 knockout of female-biased genes AeAct-4 or myo-fem in Ae. aegypti results in a flightless phenotype in female, but not male mosquitoes.PLoS neglected tropical diseases · 2020Article
- How to turn an organism into a model organism in 10 'easy' steps.The Journal of experimental biology · 2020Review
- Article
- DNA damage response and repair in perspective: Aedes aegypti, Drosophila melanogaster and Homo sapiens.Parasites & vectors · 2019Review
- Live calcium imaging of Aedes aegypti neuronal tissues reveals differential importance of chemosensory systems for life-history-specific foraging strategies.BMC neuroscience · 2019Article
- Optimization of sand fly embryo microinjection for gene editing by CRISPR/Cas9.PLoS neglected tropical diseases · 2018Article
- Germline Cas9 expression yields highly efficient genome engineering in a major worldwide disease vector,Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- The neurotranscriptome of the Aedes aegypti mosquito.BMC genomics · 2016Article
- Methods for TALEN Evaluation, Use, and Mutation Detection in the Mosquito Aedes aegypti.Methods in molecular biology (Clifton, N.J.) · 2016Article
- Genome engineering with CRISPR-Cas9 in the mosquito Aedes aegypti.Cell reports · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The Culicine mosquito, Aedes aegypti, is both a major vector of arthropod-borne viruses (arboviruses) and a genetic model organism for arbovirus transmission. TALE nucleases (TALENs), a group of artificial enzymes capable of generating site-specific DNA lesions, consist of a non-specific FokI endonuclease cleavage domain fused to an engineered DNA binding domain specific to a target site. While TALENs have become an important tool for targeted gene disruption in a variety of organisms, application to the mosquito genome is a new approach. We recently described the use of TALENs to perform heritable genetic disruptions in A. aegypti. Here, we provide detailed methods that will allow other research laboratories to capitalize on the potential of this technology for understanding mosquito gene function. We describe target site selection, transient embryo-based assays to rapidly assess TALEN activity, embryonic microinjection and downstream screening steps to identify target site mutations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.