Evidence map›Paper›PMID 24556417›Full record

ArticleJournal of controlled release : official journal of the Controlled Release Society2014

DNA nanoparticle-mediated thymulin gene therapy prevents airway remodeling in experimental allergic asthma.

Adriana L da Silva, Sabrina V Martini, Soraia C Abreu, Cynthia dos S Samary, Bruno L Diaz, Sandra Fernezlian, Vanessa Karen de Sá, Vera Luiza Capelozzi, Nicholas J Boylan, Rodolfo Gustavo Goya and 4 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of controlled release : official journal of the Controlled Release Society, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
3.6field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 59 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Tyrosine-Mutant AAV8 Vector Mediated Efficient and Safe Gene Transfer of Pigment Epithelium-Derived Factor to Mouse Lungs.Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology · 2023
    Article
  5. Review
  6. Review
  7. Review
  8. Review
  9. Review
  10. Review
  11. Review
  12. Article
  13. Article
  14. Thymus-Pineal Gland Axis: Revisiting Its Role in Human Life and Ageing.International journal of molecular sciences · 2020
    Review
  15. Article
  16. Article
  17. Application of novel nanotechnologies in asthma.Annals of translational medicine · 2020
    Article
  18. Inhalable nanotherapeutics to improve treatment efficacy for common lung diseases.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2020
    Review
  19. Article
  20. PEGylated enhanced cell penetrating peptide nanoparticles for lung gene therapy.Journal of controlled release : official journal of the Controlled Release Society · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 4 institutions in 4 countries.

Adriana L da SilvaLaboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil; Laboratory of Cellular and Molecular Physiology, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Sabrina V MartiniLaboratory of Cellular and Molecular Physiology, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Soraia C AbreuLaboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Cynthia dos S SamaryLaboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Bruno L DiazLaboratory of Immunology, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Sandra FernezlianDepartment of Pathology, School of Medicine, University of São Paulo, São Paulo, Brazil.
Vanessa Karen de SáDepartment of Pathology, School of Medicine, University of São Paulo, São Paulo, Brazil.
Vera Luiza CapelozziDepartment of Pathology, School of Medicine, University of São Paulo, São Paulo, Brazil.
Nicholas J BoylanDepartment of Chemical & Biomolecular Engineering, Johns Hopkins University, Baltimore, USA; The Center for Nanomedicine, Johns Hopkins University School of Medicine, Baltimore, USA.
Rodolfo Gustavo GoyaInstitute for Biochemical Research at La Plata (INIBIOLP), and Histology and Embryology, Faculty of Medicine, National University of La Plata, La Plata, Argentina.
Jung Soo SukThe Center for Nanomedicine, Johns Hopkins University School of Medicine, Baltimore, USA; Department of Ophthalmology, The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, USA.
Patricia R M RoccoLaboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Justin HanesDepartment of Chemical & Biomolecular Engineering, Johns Hopkins University, Baltimore, USA; The Center for Nanomedicine, Johns Hopkins University School of Medicine, Baltimore, USA; Department of Ophthalmology, The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, USA. Electronic address: hanes@jhmi.edu.
Marcelo M MoralesLaboratory of Cellular and Molecular Physiology, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil. Electronic address: mmorales@biof.ufrj.br.
Universidade Federal do Rio de Janeiro · BRJohns Hopkins University · USUniversidade de São Paulo · BRBiochemistry Research Institute of La Plata · AR

Funding

Vector CoreP01HL051811 · NHLBI · JOHNS HOPKINS UNIVERSITY · PI GUGGINO, WILLIAM B. · 1993 to 2013
$20.2M
New Gene Vectors for Cystic Fibrosis (CF): Extra- & Intracellular BarriersR01EB003558 · NIBIB · JOHNS HOPKINS UNIVERSITY · PI HANES, JUSTIN S. · 2007 to 2010
$1.4M
NHLBI NIH HHS P01 HL051811NHLBI NIH HHS P01HL51811NIBIB NIH HHS R01 EB003558NIBIB NIH HHS R01EB003558
6 · The paper itself

Abstract

Thymulin has been shown to present anti-inflammatory and anti-fibrotic properties in experimental lung diseases. We hypothesized that a biologically active thymulin analog gene, methionine serum thymus factor, delivered by highly compacted DNA nanoparticles may prevent lung inflammation and remodeling in a mouse model of allergic asthma. The DNA nanoparticles are composed of a single molecule of plasmid DNA compacted with block copolymers of poly-L-lysine and polyethylene glycol (CK30PEG), which have been found safe in a human phase I/II clinical trial. Thymulin plasmids were detected in the lungs of ovalbumin-challenged asthmatic mice up to 27days after administration of DNA nanoparticles carrying thymulin plasmids. A single dose of DNA nanoparticles carrying thymulin plasmids prevented lung inflammation, collagen deposition and smooth muscle hypertrophy in the lungs of a murine model of ovalbumin-challenged allergic asthma, leading to improved lung mechanics. In the present model of chronic allergic asthma, highly compacted DNA nanoparticles using thymulin analog gene modulated the inflammatory and remodeling processes improving lung mechanics.

Indexed as

Airway RemodelingAnimalsAsthmaDNAGenetic TherapyLungMaleMiceMice, Inbred BALB CNanoparticlesPlasmidsThymic Factor, CirculatingDNAThymic Factor, CirculatingAllergy gene therapyDNA nanoparticlesLung remodelingThymulin

Identifiers

PMID24556417
PMCPMC3992277
OpenAlexW2058389543

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.