ArticleStem cells and development2014
Generation and characterization of leukemia inhibitory factor-dependent equine induced pluripotent stem cells from adult dermal fibroblasts.
Article in Stem cells and development, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 53 citations in OpenAlex.
- Generation of equine induced pluripotent stem cells from cells of embryonic, perinatal and adult tissues.Stem cell research & therapy · 2025Article
- Reprogramming efficiency and pluripotency of mule iPSCs over its parents†.Biology of reproduction · 2023Article
- Modulation of Osteogenic Differentiation of Adipose-Derived Stromal Cells by Co-Treatment with 3, 4'-Dihydroxyflavone, U0126, and N-Acetyl Cysteine.International journal of stem cells · 2022Article
- Acquisition and maintenance of pluripotency are influenced by fibroblast growth factor, leukemia inhibitory factor, and 2i in bovine-induced pluripotent stem cells.Frontiers in cell and developmental biology · 2022Article
- iPSC Therapy for Myocardial Infarction in Large Animal Models: Land of Hope and Dreams.Biomedicines · 2021Review
- Article
- Rewinding Extinction in the Northern White Rhinoceros: Genetically Diverse Induced Pluripotent Stem Cell Bank for Genetic Rescue.Stem cells and development · 2021Article
- Perspectives of pluripotent stem cells in livestock.World journal of stem cells · 2021Review
- The use of induced pluripotent stem cells in domestic animals: a narrative review.BMC veterinary research · 2020Review
- Induced pluripotent stem cells from farm animals.Journal of animal science · 2020Review
- Genetic Signatures of Evolution of the Pluripotency Gene Regulating Network across Mammals.Genome biology and evolution · 2020Article
- Generation of induced pluripotent stem cells from large domestic animals.Stem cell research & therapy · 2020Article
- Differentiation of equine induced pluripotent stem cells into mesenchymal lineage for therapeutic use.Cell cycle (Georgetown, Tex.) · 2019Article
- Induced pluripotent stem cells throughout the animal kingdom: Availability and applications.World journal of stem cells · 2019Review
- Stem cells on regenerative and reproductive science in domestic animals.Veterinary research communications · 2019Review
- Generation and miRNA Characterization of Equine Induced Pluripotent Stem Cells Derived from Fetal and Adult Multipotent Tissues.Stem cells international · 2019Article
- Long-term expansion of primary equine keratinocytes that maintain the ability to differentiate into stratified epidermis.Stem cell research & therapy · 2018Article
- Osteoblast differentiation of equine induced pluripotent stem cells.Biology open · 2018Article
- Allogeneic Stem Cells Alter Gene Expression and Improve Healing of Distal Limb Wounds in Horses.Stem cells translational medicine · 2018Article
- Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study we have reprogrammed dermal fibroblasts from an adult female horse into equine induced pluripotent stem cells (equiPSCs). These equiPSCs are dependent only on leukemia inhibitory factor (LIF), placing them in striking contrast to previously derived equiPSCs that have been shown to be co-dependent on both LIF and basic fibroblast growth factor (bFGF). These equiPSCs have a normal karyotype and have been maintained beyond 60 passages. They possess alkaline phosphatase activity and express eqNANOG, eqOCT4, and eqTERT mRNA. Immunocytochemistry confirmed that they produce NANOG, REX1, SSEA4, TRA1-60, and TRA1-81. While our equiPSCs are LIF dependent, bFGF co-stimulates their proliferation via the PI3K/AKT pathway. EquiPSCs lack expression of eqXIST and immunostaining for H3K27me3, suggesting that during reprogramming the inactive X chromosome has likely been reactivated to generate cells that have two active X chromosomes. EquiPSCs form embryoid bodies and in vitro teratomas that contain derivatives of all three germ layers. These LIF-dependent equiPSCs likely reflect a more naive state of pluripotency than equiPSCs that are co-dependent on both LIF and bFGF and so provide a novel resource for understanding pluripotency in the horse.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.