ArticleMicrobial cell factories2014
Differential proteomic profiling reveals regulatory proteins and novel links between primary metabolism and spinosad production in Saccharopolyspora spinosa.
Article in Microbial cell factories, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 51 citations in OpenAlex.
- An efficient endogenous type I-E CRISPR-Cas genome-editing platform for producing transglutaminase inEngineering microbiology · 2026Article
- CRISPRi-mediated multigene downregulating redirects the metabolic flux to spinosad biosynthesis inSynthetic and systems biotechnology · 2025Article
- Developing a robust genome editing tool based on an endogenous type I-B CRISPR-Cas system in Saccharopolyspora spinosa.Science China. Life sciences · 2025Article
- Engineering the TetR-family transcriptional regulator XNR_0706 to enhance heterologous spinosad production inSynthetic and systems biotechnology · 2025Article
- Enhanced triacylglycerol metabolism contributes to the efficient biosynthesis of spinosad inSynthetic and systems biotechnology · 2024Article
- A TetR family transcriptional regulator, SP_2854 can affect the butenyl-spinosyn biosynthesis by regulating glucose metabolism in Saccharopolyspora pogona.Microbial cell factories · 2022Article
- The Global Regulator PhoU Positively Controls Growth and Butenyl-Spinosyn Biosynthesis inFrontiers in microbiology · 2022Article
- Deletion of a hybrid NRPS-T1PKS biosynthetic gene cluster via Latour gene knockout system in Saccharopolyspora pogona and its effect on butenyl-spinosyn biosynthesis and growth development.Microbial biotechnology · 2021Article
- Bacterioferritin: a key iron storage modulator that affects strain growth and butenyl-spinosyn biosynthesis in Saccharopolyspora pogona.Microbial cell factories · 2021Article
- Effects of acuC on the growth development and spinosad biosynthesis of Saccharopolyspora spinosa.Microbial cell factories · 2021Article
- Comparative transcriptomic analysis of two Saccharopolyspora spinosa strains reveals the relationships between primary metabolism and spinosad production.Scientific reports · 2021Article
- The Role of Subinhibitory Concentrations of Daptomycin and Tigecycline in Modulating Virulence inAntibiotics (Basel, Switzerland) · 2021Article
- Effect of the TetR family transcriptional regulator Sp1418 on the global metabolic network of Saccharopolyspora pogona.Microbial cell factories · 2020Article
- Deciphering the Metabolic Pathway Difference BetweenFrontiers in microbiology · 2020Article
- Cytosporone B as a Biological Preservative: Purification, Fungicidal Activity and Mechanism of Action againstBiomolecules · 2019Article
- Engineering of leucine-responsive regulatory protein improves spiramycin and bitespiramycin biosynthesis.Microbial cell factories · 2019Article
- High Level of Spinosad Production in the Heterologous Host Saccharopolyspora erythraea.Applied and environmental microbiology · 2016Article
- Engineering microbial hosts for production of bacterial natural products.Natural product reports · 2016Review
- Elucidating the molecular physiology of lantibiotic NAI-107 production in Microbispora ATCC-PTA-5024.BMC genomics · 2016Article
- Proteomic analysis of the influence of Cu(2+) on the crystal protein production of Bacillus thuringiensis X022.Microbial cell factories · 2015Article
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSaccharopolyspora spinosa is an important producer of antibiotic spinosad with clarified biosynthesis pathway but its complex regulation networks associated with primary metabolism and secondary metabolites production almost have never been concerned or studied before. The proteomic analysis of a novel Saccharopolyspora spinosa CCTCC M206084 was performed and aimed to provide a global profile of regulatory proteins.
resultsTwo-dimensional-liquid chromatography-tandem mass spectrometry (LC-MS/MS) identified 1090, 1166, 701, and 509 proteins from four phases respectively, i.e., the logarithmic growth phase (T1), early stationary phase (T2), late stationary phase (T3), and decline phase (T4). Among the identified proteins, 1579 were unique to the S. spinosa proteome, including almost all the enzymes for spinosad biosynthesis. Trends in protein expression over the various time phases were deduced from using the modified protein abundance index (PAI), revealed the importance of stress pathway proteins and other global regulatory network proteins during spinosad biosynthesis. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis followed by one-dimensional LC-MS/MS identification revealed similar trend of protein expression from four phases with the results of semi-quantification by PAI. qRT-PCR analysis revealed that 6 different expressed genes showed a positive correlation between changes at translational and transcriptional expression level. Expression of three proteins that likely promote spinosad biosynthesis, namely, 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase (MHSM), glutamine synthetase (GS) and cyclic nucleotide-binding domain-containing protein (CNDP) was validated by western blot, which confirmed the results of proteomic analysis.
conclusionsThis study is the first systematic analysis of the S. spinosa proteome during fermentation and its valuable proteomic data of regulatory proteins may be used to enhance the production yield of spinosad in future studies.
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