ArticleThe Analyst2014
Live-cell vibrational imaging of choline metabolites by stimulated Raman scattering coupled with isotope-based metabolic labeling.
Article in The Analyst, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- The Next Frontier in Vibrational Imaging: Chemically Designed Raman Probes Pass the Spectral Barrier.JACS Au · 2026Review
- Stimulated Raman Scattering Imaging Enabled Biomarker Discovery for Precision Medicine.Chemical & biomedical imaging · 2025Review
- Recent advances in Raman probes for multiplexed bioimaging.Inflammation and regeneration · 2025Review
- Illuminating life processes by vibrational probes.Nature methods · 2025Review
- Synthetic Lipid Biology.Chemical reviews · 2025Review
- An Activity-Based Sensing Approach to Multiplex Mapping of Labile Copper Pools by Stimulated Raman Scattering.Journal of the American Chemical Society · 2024Article
- Fast Real-Time Brain Tumor Detection Based on Stimulated Raman Histology and Self-Supervised Deep Learning Model.Journal of imaging informatics in medicine · 2024Article
- Seeing is Believing: Developing Multimodal Metabolic Insights at the Molecular Level.ACS central science · 2024Review
- Biomedical applications, perspectives and tag design concepts in the cell - silent Raman window.RSC chemical biology · 2024Review
- Recent Advances in Enhancement of Raman Scattering Intensity for Biological Applications.Chemical & biomedical imaging · 2023Review
- Raman Spectroscopy as a Tool to Study the Pathophysiology of Brain Diseases.International journal of molecular sciences · 2023Review
- Imaging the uptake of deuterated methionine inFrontiers in chemistry · 2023Article
- Raman Spectroscopy for Chemical Biology Research.Journal of the American Chemical Society · 2022Review
- Bringing Vibrational Imaging to Chemical Biology with Molecular Probes.ACS chemical biology · 2022Article
- Direct visualization of general anesthetic propofol on neurons by stimulated Raman scattering microscopy.iScience · 2022Article
- Toward Raman Subcellular Imaging of Endothelial Dysfunction.Journal of medicinal chemistry · 2021Review
- Advances in stimulated Raman scattering imaging for tissues and animals.Quantitative imaging in medicine and surgery · 2021Review
- Quinine copolymer reporters promote efficient intracellular DNA delivery and illuminate a protein-induced unpackaging mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Mammalian cell and tissue imaging using Raman and coherent Raman microscopy.Wiley interdisciplinary reviews. Systems biology and medicine · 2020Review
- Molecular imaging of extracellular vesicles in vitro via Raman metabolic labelling.Journal of materials chemistry. B · 2020Article
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Authors and funding
5 authors.
Funding
Abstract
Choline is a small molecule that occupies a key position in the biochemistry of all living organisms. Recent studies have strongly implicated choline metabolites in cancer, atherosclerosis and nervous system development. To detect choline and its metabolites, existing physical methods such as magnetic resonance spectroscopy and positron emission tomography are often limited by the poor spatial resolution and substantial radiation dose. Fluorescence imaging, although with submicrometer resolution, requires introduction of bulky fluorophores and thus is difficult in labeling the small choline molecule. By combining the emerging bond-selective stimulated Raman scattering microscopy with metabolic incorporation of deuterated choline, herein we have achieved high resolution imaging of choline-containing metabolites in living mammalian cell lines, primary hippocampal neurons and the multicellular organism C. elegans. Different subcellular distributions of choline metabolites are observed between cancer cells and non-cancer cells, which may reveal a functional difference in the choline metabolism and lipid-mediated signaling events. In neurons, choline incorporation is visualized within both soma and neurites, where choline metabolites are more evenly distributed compared to proteins. Furthermore, choline localization is also observed in the pharynx region of C. elegans larvae, consistent with its organogenesis mechanism. These applications demonstrate the potential of isotope-based stimulated Raman scattering microscopy for future choline-related disease detection and development monitoring in vivo.
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Registered trials
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