ArticleThe Biochemical journal1988
The preparation and characterization of monoclonal antibodies to human complement component C8 and their use in purification of C8 and C8 subunits.
Article in The Biochemical journal, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 34 citations in OpenAlex.
- Role of the complement system in kidney cell death induced by Loxosceles venom Sphingomyelinases D.Archives of toxicology · 2024Article
- Structure of human complement C8, a precursor to membrane attack.Journal of molecular biology · 2011Article
- Characterization of the mouse analogues of CD59 using novel monoclonal antibodies: tissue distribution and functional comparison.Immunology · 2003Article
- Mechanism of induction of complement susceptibility of erythrocytes by spider and bacterial sphingomyelinases.Immunology · 2002Article
- Streptococcal inhibitor of complement (SIC) inhibits the membrane attack complex by preventing uptake of C567 onto cell membranes.Immunology · 2001Article
- Production and functional analysis of rat CD59 and chimeric CD59-Crry as active soluble proteins in Pichia pastoris.Immunology · 2000Article
- Characterization in vitro and in vivo of the pig analogue of human CD59 using new monoclonal antibodies.Immunology · 1998Article
- Expression of rat CD59: functional analysis confirms lack of species selectivity and reveals that glycosylation is not required for function.Immunology · 1997Article
- Binding of human and rat CD59 to the terminal complement complexes.Immunology · 1997Article
- A component of the medicinal herb ephedra blocks activation in the classical and alternative pathways of complement.Clinical and experimental immunology · 1995Article
- Identification of a lysosomal protein causing lipid transfer, using a fluorescence assay designed to monitor membrane fusion between rat liver endosomes and lysosomes.The Biochemical journal · 1995Article
- Enhanced complement susceptibility of avidin-biotin-treated human erythrocytes is a consequence of neutralization of the complement regulators CD59 and decay accelerating factor.The Biochemical journal · 1995Article
- Lysosomes can fuse with a late endosomal compartment in a cell-free system from rat liver.The Journal of cell biology · 1994Article
- Physiologic relevance of the membrane attack complex inhibitory protein CD59 in human seminal plasma: CD59 is present on extracellular organelles (prostasomes), binds cell membranes, and inhibits complement-mediated lysis.The Journal of experimental medicine · 1993Article
- Membrane attack complex (MAC)-mediated damage to spermatozoa: protection of the cells by the presence on their membranes of MAC inhibitory proteins.Immunology · 1992Article
- Human rheumatoid synovial cell stimulation by the membrane attack complex and other pore-forming toxins in vitro: the role of calcium in cell activation.Immunology · 1990Article
- Thyroid follicular cell function after non-lethal complement membrane attack.Clinical and experimental immunology · 1990Article
- Human protectin (CD59), an 18,000-20,000 MW complement lysis restricting factor, inhibits C5b-8 catalysed insertion of C9 into lipid bilayers.Immunology · 1990Article
- Stimulation of human rheumatoid synovial cells by non-lethal complement membrane attack.Immunology · 1990Article
- Isolation and characterization of a membrane-attack-complex-inhibiting protein present in human serum and other biological fluids.The Biochemical journal · 1990Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. Ten mouse monoclonal antibodies to human complement component C8 were prepared. It was found that six of these antibodies reacted with the alpha-subunit, two with the beta-subunit and two with the gamma-subunit, when assessed by immunoblotting after separation of C8 subunits by SDS/polyacrylamide-gel electrophoresis. 2. Epitope analysis of the ten monoclonal antibodies in a competitive binding assay showed that the six antibodies to the alpha-subunit could be classified in four overlapping epitope groups. The antibodies to the beta- and gamma-subunits bound to a single antigenic site on each, but also cross-reacted with the antigenic sites on the alpha-subunit. 3. Monoclonal anti-C8 immunoaffinity columns were used to purify C8 from fresh human plasma and to prepare C8-depleted serum. Immunoaffinity purified C8 was biologically active when assessed by using haemolysis assays of sheep and rabbit erythrocytes. 4. Salt elution was used to purify either alpha gamma- or beta-subunits when C8 was respectively bound to an anti-beta or anti-alpha immunoaffinity column. The purified subunits reconstituted C8-depleted serum when added together in a haemolysis assay.
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