Evidence map›Paper›PMID 24554848›Full record

ArticlePhysiology and molecular biology of plants : an international journal of functional plant biology2014

Analysis of Jatropha curcas transcriptome for oil enhancement and genic markers.

Atul Grover, Maya Kumari, Sadhana Singh, Shivender Singh Rathode, Sanjay Mohan Gupta, Pankaj Pandey, Sween Gilotra, Devender Kumar, Mohommad Arif, Zakwan Ahmed

Abstract read
In one paragraph

Article in Physiology and molecular biology of plants : an international journal of functional plant biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

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0cells of the map it votes in
5citing papers in PubMed
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1 · What the graph read from it

What it found

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3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Atul GroverBiotechnology Division, Defence Institute of Bio-Energy Research, Goraparao, P.O. Arjunpur, Haldwani, 263 139 India.
Maya KumariBiotechnology Division, Defence Institute of Bio-Energy Research, Goraparao, P.O. Arjunpur, Haldwani, 263 139 India.
Sadhana SinghBiotechnology Division, Defence Institute of Bio-Energy Research, Goraparao, P.O. Arjunpur, Haldwani, 263 139 India.
Shivender Singh RathodeBiotechnology Division, Defence Institute of Bio-Energy Research, Goraparao, P.O. Arjunpur, Haldwani, 263 139 India.
Sanjay Mohan GuptaBiotechnology Division, Defence Institute of Bio-Energy Research, Goraparao, P.O. Arjunpur, Haldwani, 263 139 India.
Pankaj PandeyBiotechnology Division, Defence Institute of Bio-Energy Research, Goraparao, P.O. Arjunpur, Haldwani, 263 139 India.
Sween GilotraDepartment of Biotechnology, Indian Institute of Technology, Guwahati, India.
Devender KumarBiotechnology Division, Defence Institute of Bio-Energy Research, Goraparao, P.O. Arjunpur, Haldwani, 263 139 India.
Mohommad ArifBiotechnology Division, Defence Institute of Bio-Energy Research, Goraparao, P.O. Arjunpur, Haldwani, 263 139 India.
Zakwan AhmedBiotechnology Division, Defence Institute of Bio-Energy Research, Goraparao, P.O. Arjunpur, Haldwani, 263 139 India ; Directorate of Management Services, Defence Research and Development Organization, DRDO Bhawan, New Delhi, 110011 India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Oil-rich seeds of Jatropha curcas are being focussed as a source of bio-diesel. However, prior to its industrial use, a lot of crop improvement efforts are required in Jatropha. Availability of a large number of EST sequences of Jatropha in public domain allow identification of candidate genes for several agronomic characters including oil content in seeds. Here, we have analysed 42,477 ESTs of Jatropha spanning 22.9 Mbp for microsatellites and fatty acid metabolism related sequences. Unigene sequences were built using CAP 3 programme resulted in 12,358 contigs and 5,730 singlets. Nearly, 8 % unigenes showed presence of microsatellites, slightly over-represented compared to their occurrence in ESTs. Most of the microsatellites were either di- or tri-nucleotide repeats, while other categories of tetra-, penta- and hexa-nucleotide repeats together constituted ~4 % of total microsatellites. Assessment of functional relevance of unigenes was carried out using Blast2GO using its default settings. The overall sequence similarity level against sequences in 'nr' database was >80 %. A total of 931 sequences that participated in any of the pathways related to fatty acid or lipid metabolism were found at GO level 6. Among these, GO terms "Fatty acid metabolic process" and "Fatty acid biosynthetic process" were most over-represented. Overall, our work has due relevance in identifying molecular markers for the candidate genes for oil content in Jatropha seeds, and will prove to be an important reference for further studies for identification of trait specific markers in Jatropha.

Indexed as

ESTsFatty acidsGene ontologyJatropha curcasMicrosatellitesUnigenes

Identifiers

PMID24554848
PMCPMC3925477

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.