ArticleJournal of clinical microbiology2014
Development and validation of an in-house database for matrix-assisted laser desorption ionization-time of flight mass spectrometry-based yeast identification using a fast protein extraction procedure.
Article in Journal of clinical microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- The rise ofEuro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2025Article
- Genetic Epidemiology and Resistance Investigations of Clinical Yeasts in Alexandria, Egypt.Pathogens (Basel, Switzerland) · 2025Article
- Unveiling Lodderomyces elongisporus as an Emerging Yeast Pathogen: A Holistic Approach to Microbiological Diagnostic Strategies.Mycopathologia · 2024Review
- The yeast genus Tardiomyces gen. nov. with one new species and two new combinations.Infection · 2024Article
- Evaluation of Autof MS2600 and MBT Smart MALDI-TOF MS Systems for Routine Identification of Clinical Bacteria and Yeasts.Microorganisms · 2024Article
- Application of Novel Short Tandem Repeat Typing forMicroorganisms · 2023Article
- Short Tandem Repeat Genotyping and Antifungal Susceptibility Testing of Latin AmericanJournal of fungi (Basel, Switzerland) · 2023Article
- Development of a Multiplex PCR Short Tandem Repeat Typing Scheme for Candida krusei.Journal of clinical microbiology · 2022Article
- Are We Ready for NosocomialFrontiers in cellular and infection microbiology · 2021Article
- Implementation of MALDI-TOF Mass Spectrometry and Peak Analysis: Application to the Discrimination ofJournal of fungi (Basel, Switzerland) · 2020Article
- Molecular phylogenetic and matrix-assisted laser desorption ionization time-of-flight mass spectrometry identification of isolates from horses identified asJournal of equine science · 2020Article
- Pan-Echinocandin-ResistantJournal of fungi (Basel, Switzerland) · 2020Article
- Different detection capabilities by mycological media for Candida isolates from mono- or dual-species cultures.PloS one · 2020Article
- Genotyping Reveals High Clonal Diversity and Widespread Genotypes ofFrontiers in cellular and infection microbiology · 2020Article
- Prevalence and Clonal Distribution of Azole-ResistantFrontiers in cellular and infection microbiology · 2020Article
- Identification of microbiota in peri-implantitis pockets by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.Scientific reports · 2019Article
- A Moldy Application of MALDI: MALDI-ToF Mass Spectrometry for Fungal Identification.Journal of fungi (Basel, Switzerland) · 2019Review
- BIOF-HILO Assay: A New MALDI-TOF Mass Spectrometry Based Method for Discriminating Between High- and Low-Biofilm-ProducingFrontiers in microbiology · 2019Article
- Performance evaluation of the (1,3)-β-D-glucan detection assay in non-intensive care unit adult patients.Infection and drug resistance · 2019Article
- Fatal fulminant cryptococcemia complicating sarcoidosis: Is it to be expected?Medical mycology case reports · 2018Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In recent studies evaluating the usefulness of the matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)-based identification of yeasts for the routine diagnosis of fungal infections, preanalytical sample processing has emerged as a critical step for reliable MALDI-TOF MS outcomes, especially when the Bruker Daltonics Biotyper software was used. In addition, inadequate results often occurred due to discrepancies between the methods used for clinical testing and database construction. Therefore, we created an in-house MALDI-TOF MS library using the spectra from 156 reference and clinical yeast isolates (48 species in 11 genera), which were generated with a fast sample preparation procedure. After a retrospective validation study, our database was evaluated on 4,232 yeasts routinely isolated during a 6-month period and fast prepared for MALDI-TOF MS analysis. Thus, 4,209 (99.5%) of the isolates were successfully identified to the species level (with scores of ≥2.0), with 1,676 (39.6%) having scores of >2.3. For the remaining 23 (0.5%) isolates, no reliable identification (with scores of <1.7) was obtained. Interestingly, these isolates were almost always from species uniquely represented or not included in the database. As the MALDI-TOF MS results were, except for 23 isolates, validated without additional phenotypic or molecular tests, our proposed strategy can enhance the rapidity and accuracy of MALDI-TOF MS in identifying medically important yeast species. However, while continuous updating of our database will be necessary to enrich it with more strains/species of new and emerging yeasts, the present in-house MALDI-TOF MS library can be made publicly available for future multicenter studies.
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