ArticleThe Journal of biological chemistry2014
High oxygen condition facilitates the differentiation of mouse and human pluripotent stem cells into pancreatic progenitors and insulin-producing cells.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 48 citations in OpenAlex.
- Review
- Early hypoxia treatment and oxygen-concentration alteration promote the retinal progenitor proliferation and ganglion cell maturation in human retinal organoid development.Stem cell research & therapy · 2026Article
- Oxygen dynamics and delivery strategies to enhance beta cell replacement therapy.American journal of physiology. Cell physiology · 2025Review
- Cell Seeding Strategy Influences Metabolism and Differentiation Potency of Human Induced Pluripotent Stem Cells Into Pancreatic Progenitors.Biotechnology journal · 2025Article
- Mitochondria and pluripotency: from established models to emerging roles in adult stem cells.Frontiers in bioengineering and biotechnology · 2025Review
- Identifying and optimizing critical process parameters for large-scale manufacturing of iPSC derived insulin-producing β-cells.Stem cell research & therapy · 2024Article
- Oxygenated Scaffolds for Pancreatic Endocrine Differentiation from Induced Pluripotent Stem Cells.Advanced healthcare materials · 2024Article
- Stress Factors as Possible Regulators of Pluripotent Stem Cell Survival and Differentiation.Biology · 2023Review
- Integrating Oxygen and 3D Cell Culture System: A Simple Tool to Elucidate the Cell Fate Decision of hiPSCs.International journal of molecular sciences · 2022Article
- Optimization of an OScientific reports · 2022Article
- Adapting Physiology in Functional Human Islet Organogenesis.Frontiers in cell and developmental biology · 2022Article
- Mouse Pluripotent Stem Cell Differentiation Under Physiological Oxygen Reduces Residual Teratomas.Cellular and molecular bioengineering · 2021Article
- Oxygen as a Master Regulator of Human Pluripotent Stem Cell Function and Metabolism.Journal of personalized medicine · 2021Review
- Molecular mechanisms of transcription factor mediated cell reprogramming: conversion of liver to pancreas.Biochemical Society transactions · 2021Review
- Response of Pluripotent Stem Cells to Environmental Stress and Its Application for Directed Differentiation.Biology · 2021Review
- Recent Advances in the Generation of β-Cells from Induced Pluripotent Stem Cells as a Potential Cure for Diabetes Mellitus.Advances in experimental medicine and biology · 2021Review
- Emerging routes to the generation of functional β-cells for diabetes mellitus cell therapy.Nature reviews. Endocrinology · 2020Review
- Developmentally-Inspired Biomimetic Culture Models to Produce Functional Islet-Like Cells From Pluripotent Precursors.Frontiers in bioengineering and biotechnology · 2020Review
- In vitro differentiation of human multilineage differentiating stress-enduring (Muse) cells into insulin producing cells.Journal, genetic engineering & biotechnology · 2018Article
- Epigenetic Control of Endocrine Pancreas DifferentiationFrontiers in cell and developmental biology · 2018Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pluripotent stem cells have potential applications in regenerative medicine for diabetes. Differentiation of stem cells into insulin-producing cells has been achieved using various protocols. However, both the efficiency of the method and potency of differentiated cells are insufficient. Oxygen tension, the partial pressure of oxygen, has been shown to regulate the embryonic development of several organs, including pancreatic β-cells. In this study, we tried to establish an effective method for the differentiation of induced pluripotent stem cells (iPSCs) into insulin-producing cells by culturing under high oxygen (O2) conditions. Treatment with a high O2 condition in the early stage of differentiation increased insulin-positive cells at the terminus of differentiation. We found that a high O2 condition repressed Notch-dependent gene Hes1 expression and increased Ngn3 expression at the stage of pancreatic progenitors. This effect was caused by inhibition of hypoxia-inducible factor-1α protein level. Moreover, a high O2 condition activated Wnt signaling. Optimal stage-specific treatment with a high O2 condition resulted in a significant increase in insulin production in both mouse embryonic stem cells and human iPSCs and yielded populations containing up to 10% C-peptide-positive cells in human iPSCs. These results suggest that culturing in a high O2 condition at a specific stage is useful for the efficient generation of insulin-producing cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.