ArticleJournal of virology2014
Identification and characterization of a proteolytically primed form of the murine coronavirus spike proteins after fusion with the target cell.
Article in Journal of virology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 28 citations in OpenAlex.
- TMPRSS2-mediated coronavirus spike activation and inhibition.Nature structural & molecular biology · 2026Article
- Review
- SARS-CoV-2 spike engagement of ACE2 primes S2' site cleavage and fusion initiation.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Cell Entry of Animal Coronaviruses.Viruses · 2021Review
- The role of high cholesterol in age-related COVID19 lethality.bioRxiv : the preprint server for biology · 2021Article
- Article
- Biochemical Characterization of Middle East Respiratory Syndrome Coronavirus Spike Protein Proteolytic Processing.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Biochemical Analysis of Coronavirus Spike Glycoprotein Conformational Intermediates during Membrane Fusion.Journal of virology · 2019Article
- Tetraspanins: Architects of Viral Entry and Exit Platforms.Journal of virology · 2019Review
- Molecular dynamics of Middle East Respiratory Syndrome Coronavirus (MERS CoV) fusion heptad repeat trimers.Computational biology and chemistry · 2018Article
- Kinetic analysis of the influenza A virus HA/NA balance reveals contribution of NA to virus-receptor binding and NA-dependent rolling on receptor-containing surfaces.PLoS pathogens · 2018Article
- Identification of H209 as Essential for pH 8-Triggered Receptor-Independent Syncytium Formation by S Protein of Mouse Hepatitis Virus A59.Journal of virology · 2018Article
- Neurovirulent Murine Coronavirus JHM.SD Uses Cellular Zinc Metalloproteases for Virus Entry and Cell-Cell Fusion.Journal of virology · 2017Article
- Characterization of an Immunodominant Epitope in the Endodomain of the Coronavirus Membrane Protein.Viruses · 2016Article
- Cellular entry of the porcine epidemic diarrhea virus.Virus research · 2016Review
- Proteolytic processing of Middle East respiratory syndrome coronavirus spikes expands virus tropism.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- An siRNA screen for ATG protein depletion reveals the extent of the unconventional functions of the autophagy proteome in virus replication.The Journal of cell biology · 2016Article
- Fusion of Enveloped Viruses in Endosomes.Traffic (Copenhagen, Denmark) · 2016Review
- Genotyping coronaviruses associated with feline infectious peritonitis.The Journal of general virology · 2015Article
- Host cell proteases: Critical determinants of coronavirus tropism and pathogenesis.Virus research · 2015Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
unlabelledEnveloped viruses carry highly specialized glycoproteins that catalyze membrane fusion under strict spatial and temporal control. To prevent premature activation after biosynthesis, viral class I fusion proteins adopt a locked conformation and require proteolytic cleavage to render them fusion-ready. This priming step may occur during virus exit from the infected cell, in the extracellular milieu or during entry at or in the next target cell. Proteolytic processing of coronavirus spike (S) fusion proteins during virus entry has been suggested but not yet formally demonstrated, while the nature and functionality of the resulting subunit is still unclear. We used a prototype coronavirus--mouse hepatitis virus (MHV)--to develop a conditional biotinylation assay that enables the specific identification and biochemical characterization of viral S proteins on virions that mediated membrane fusion with the target cell. We demonstrate that MHV S proteins are indeed cleaved upon virus endocytosis, and we identify a novel processing product S2* with characteristics of a fusion-active subunit. The precise cleavage site and the enzymes involved remain to be elucidated. IMPORTANCE: Virus entry determines the tropism and is a crucial step in the virus life cycle. We developed an approach to characterize structural components of virus particles after entering new target cells. A prototype coronavirus was used to illustrate how the virus fusion machinery can be controlled.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.