ArticleRevista do Instituto de Medicina Tropical de Sao Paulo
Evaluation of four different DNA extraction methods in coagulase-negative staphylococci clinical isolates.
Article in Revista do Instituto de Medicina Tropical de Sao Paulo. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 45 citations in OpenAlex.
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- Relationship of OqxAB efflux pump to antibiotic resistance, mainly fluoroquinolones inIranian journal of basic medical sciences · 2023Article
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- Pathogenic bacteria in cheese, raw and pasteurised milk.Veterinary medicine and science · 2021Article
- Persister cells as a possible cause of antibiotic therapy failure inJGH open : an open access journal of gastroenterology and hepatology · 2021Article
- Antimicrobial Usage Factors and Resistance Profiles of Shiga Toxin-ProducingFrontiers in veterinary science · 2020Article
- Phenotypic and Genotypic Antimicrobial Resistance in Non-O157 Shiga Toxin-ProducingFrontiers in veterinary science · 2020Article
- Antibiotic Resistance and Virulence Gene Characteristics of Methicillin-ResistantInternational journal of microbiology · 2020Article
- Virulence Genes, Shiga Toxin Subtypes, Serogroups, and Clonal Relationship of Shiga Toxin-ProducingAnimals : an open access journal from MDPI · 2019Article
- In vitro antibacterial effect of deconex and sodium hypochlorite against bacterial taxa isolated from dental units.Infection and drug resistance · 2019Article
- Electronic Properties of Synthetic Shrimp Pathogens-derived DNA Schottky Diodes.Scientific reports · 2018Article
- Distribution ofJournal of clinical and diagnostic research : JCDR · 2017Article
- A simple and efficient total genomic DNA extraction method for individual zooplankton.SpringerPlus · 2016Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Currently there are several methods to extract bacterial DNA based on different principles. However, the amount and the quality of the DNA obtained by each one of those methods is highly variable and microorganism dependent, as illustrated by coagulase-negative staphylococci (CoNS) which have a thick cell wall that is difficult to lyse. This study was designed to compare the quality and the amount of CoNS DNA, extracted by four different techniques: two in-house protocols and two commercial kits. DNA amount and quality determination was performed through spectrophotometry. The extracted DNA was also analyzed using agarose gel electrophoresis and by PCR. 267 isolates of CoNS were used in this study. The column method and thermal lyses showed better results with regard to DNA quality (mean ratio of A260/280 = 1.95) and average concentration of DNA (), respectively. All four methods tested provided appropriate DNA for PCR amplification, but with different yields. DNA quality is important since it allows the application of a large number of molecular biology techniques, and also it's storage for a longer period of time. In this sense the extraction method based on an extraction column presented the best results for CoNS.
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