Evidence map›Paper›PMID 24553467›Full record

ArticleThe ISME journal2014

Phylogenetic distribution of three pathways for propionate production within the human gut microbiota.

Nicole Reichardt, Sylvia H Duncan, Pauline Young, Alvaro Belenguer, Carol McWilliam Leitch, Karen P Scott, Harry J Flint, Petra Louis

Erratum issuedOpen access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The ISME journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 673 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
673citing papers in PubMed, 3 pooled it
19.5field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

673 citing papers in PubMed, 3 syntheses or guidelines pooled it, 1,225 citations in OpenAlex.

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613 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

  • Erratum issued
5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Nicole Reichardt1] Rowett Institute of Nutrition and Health, University of Aberdeen, Bucksburn, UK [2] Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
Sylvia H DuncanRowett Institute of Nutrition and Health, University of Aberdeen, Bucksburn, UK.
Pauline YoungRowett Institute of Nutrition and Health, University of Aberdeen, Bucksburn, UK.
Alvaro BelenguerRowett Institute of Nutrition and Health, University of Aberdeen, Bucksburn, UK.
Carol McWilliam LeitchRowett Institute of Nutrition and Health, University of Aberdeen, Bucksburn, UK.
Karen P ScottRowett Institute of Nutrition and Health, University of Aberdeen, Bucksburn, UK.
Harry J FlintRowett Institute of Nutrition and Health, University of Aberdeen, Bucksburn, UK.
Petra LouisRowett Institute of Nutrition and Health, University of Aberdeen, Bucksburn, UK.
University of Aberdeen · GBUniversity of Glasgow · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Propionate is produced in the human large intestine by microbial fermentation and may help maintain human health. We have examined the distribution of three different pathways used by bacteria for propionate formation using genomic and metagenomic analysis of the human gut microbiota and by designing degenerate primer sets for the detection of diagnostic genes for these pathways. Degenerate primers for the acrylate pathway (detecting the lcdA gene, encoding lactoyl-CoA dehydratase) together with metagenomic mining revealed that this pathway is restricted to only a few human colonic species within the Lachnospiraceae and Negativicutes. The operation of this pathway for lactate utilisation in Coprococcus catus (Lachnospiraceae) was confirmed using stable isotope labelling. The propanediol pathway that processes deoxy sugars such as fucose and rhamnose was more abundant within the Lachnospiraceae (based on the pduP gene, which encodes propionaldehyde dehydrogenase), occurring in relatives of Ruminococcus obeum and in Roseburia inulinivorans. The dominant source of propionate from hexose sugars, however, was concluded to be the succinate pathway, as indicated by the widespread distribution of the mmdA gene that encodes methylmalonyl-CoA decarboxylase in the Bacteroidetes and in many Negativicutes. In general, the capacity to produce propionate or butyrate from hexose sugars resided in different species, although two species of Lachnospiraceae (C. catus and R. inulinivorans) are now known to be able to switch from butyrate to propionate production on different substrates. A better understanding of the microbial ecology of short-chain fatty acid formation may allow modulation of propionate formation by the human gut microbiota.

Indexed as

MicrobiotaAcrylatesBacteriaFermentationGastrointestinal TractGram-Positive BacteriaHumansPhylogenyPropionatesPropylene GlycolsSuccinatesAcrylatesPropionatesPropylene GlycolsSuccinates

Identifiers

PMID24553467
PMCPMC4030238
OpenAlexW1990590570

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.