ArticleProceedings. Biological sciences2014
Generation of mastitis resistance in cows by targeting human lysozyme gene to β-casein locus using zinc-finger nucleases.
Article in Proceedings. Biological sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 101 citations in OpenAlex.
- Transient Expression of Hen Egg White Lysozyme (EWL) inLife (Basel, Switzerland) · 2025Article
- Generation of Anti-Mastitis Gene-Edited Dairy Goats with Enhancing Lysozyme Expression by Inflammatory Regulatory Sequence using ISDra2-TnpB System.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Current status and future of gene engineering in livestock.BMB reports · 2024Review
- Gene editing in livestock: innovations and applications.Animal reproduction · 2024Article
- Outlook on genome editing application to cattle.Journal of veterinary science · 2024Review
- Perspectives in Genome-Editing Techniques for Livestock.Animals : an open access journal from MDPI · 2023Review
- Biomimetic natural biomaterials for tissue engineering and regenerative medicine: new biosynthesis methods, recent advances, and emerging applications.Military Medical Research · 2023Review
- Transgenesis and Genome Engineering: A Historical Review.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Emerging Roles of Noncoding RNAs in Bovine Mastitis Diseases.Pathogens (Basel, Switzerland) · 2022Review
- Application of Gene Editing Technology in Resistance Breeding of Livestock.Life (Basel, Switzerland) · 2022Review
- Generation of genome-edited dogs by somatic cell nuclear transfer.BMC biotechnology · 2022Article
- Genetic modifications associated with sustainability aspects for sustainable developments.Bioengineered · 2022Review
- Cows as Bioreactors for the Production of Nutritionally and Biomedically Significant Proteins.Advances in experimental medicine and biology · 2022Article
- Secretion of IFN-γ by Transgenic Mammary Epithelial CellsFrontiers in veterinary science · 2022Article
- Historical DNA Manipulation Overview.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Advances and Perspectives in the Application of CRISPR-Cas9 in Livestock.Molecular biotechnology · 2021Review
- Impact of CRISPR-Cas9-Based Genome Engineering in Farm Animals.Veterinary sciences · 2021Review
- Genomic Analysis, Progress and Future Perspectives in Dairy Cattle Selection: A Review.Animals : an open access journal from MDPI · 2021Review
- Opportunities and Challenges for Improving the Productivity of Swamp Buffaloes in Southeastern Asia.Frontiers in genetics · 2021Review
- Histone deacetylases inhibitor and RAD51 recombinase increase transcription activator-like effector nucleases-mediated homologous recombination on the bovine β-casein gene locus.Asian-Australasian journal of animal sciences · 2020Article
Corrections and comments
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mastitis costs the dairy industry billions of dollars annually and is the most consequential disease of dairy cattle. Transgenic cows secreting an antimicrobial peptide demonstrated resistance to mastitis. The combination of somatic cell gene targeting and nuclear transfer provides a powerful method to produce transgenic animals. Recent studies found that a precisely placed double-strand break induced by engineered zinc-finger nucleases (ZFNs) stimulated the integration of exogenous DNA stretches into a pre-determined genomic location, resulting in high-efficiency site-specific gene addition. Here, we used ZFNs to target human lysozyme (hLYZ) gene to bovine β-casein locus, resulting in hLYZ knock-in of approximately 1% of ZFN-treated bovine fetal fibroblasts (BFFs). Gene-targeted fibroblast cell clones were screened by junction PCR amplification and Southern blot analysis. Gene-targeted BFFs were used in somatic cell nuclear transfer. In vitro assays demonstrated that the milk secreted by transgenic cows had the ability to kill Staphylococcus aureus. We report the production of cloned cows carrying human lysozyme gene knock-in β-casein locus using ZFNs. Our findings open a unique avenue for the creation of transgenic cows from genetic engineering by providing a viable tool for enhancing resistance to disease and improving the health and welfare of livestock.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.