ArticleClinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology2014
Hypoallergenic derivatives of Fel d 1 obtained by rational reassembly for allergy vaccination and tolerance induction.
Article in Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 36 citations in OpenAlex.
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- Studies on HBcAg-rBlo t 5-21 Fusion Protein Vaccine That AlleviatesJournal of inflammation research · 2022Article
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- Molecular diagnosis in cat allergy.World journal of methodology · 2021Review
- Egg Allergy: Diagnosis and Immunotherapy.International journal of molecular sciences · 2020Review
- A hybrid of two major Blomia tropicalis allergens as an allergy vaccine candidate.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2020Article
- An Engineered Hybrid Protein fromInternational journal of molecular sciences · 2019Article
- Der p 5 allergen from house dust mite: first epitope mapping of rabbit IgG blocking antibodies.New microbes and new infections · 2019Article
- Allergic phenotypes in adult patients with atopic dermatitis, determined with the ISAC test (ImmunoCAP ISAC).Postepy dermatologii i alergologii · 2018Article
- Next-Generation of Allergen-Specific Immunotherapies: Molecular Approaches.Current allergy and asthma reports · 2018Review
- Important and specific role for basophils in acute allergic reactions.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2018Review
- Modified Allergens for Immunotherapy.Current allergy and asthma reports · 2018Review
- An update on molecular cat allergens: Fel d 1 and what else? Chapter 1: Fel d 1, the major cat allergen.Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology · 2018Review
- Clustering of conformational IgE epitopes on the major dog allergen Can f 1.Scientific reports · 2017Article
- Single recombinant and purified major allergens and peptides: How they are made and how they change allergy diagnosis and treatment.Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology · 2017Review
- Hypoallergenic Variant of the Major Egg White Allergen Gal d 1 Produced by Disruption of Cysteine Bridges.Nutrients · 2017Article
- Recombinant Allergens in Structural Biology, Diagnosis, and Immunotherapy.International archives of allergy and immunology · 2017Review
- The Evolution of Human Basophil Biology from Neglect towards Understanding of Their Immune Functions.BioMed research international · 2016Review
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Authors and funding
13 authors at 3 institutions in 2 countries.
Funding
Abstract
BACKGROUND AND
objectiveThe major cat allergen Fel d 1 represents one of the most important respiratory allergens. Aim of this study was to engineer recombinant Fel d 1 derivatives with reduced IgE reactivity and preserved T cell epitopes for vaccination and tolerance induction.
methodsSeven recombinant mosaic proteins were generated by reassembly of non-IgE-reactive peptides of Fel d 1 which contained the sequence elements for induction of allergen-specific blocking IgG antibodies and T cell epitopes. Mosaic proteins were expressed in Escherichia coli using codon-optimized synthetic genes and compared with Fel d 1 regarding structural fold by circular dichroism, IgE-binding capacity, activation of allergic patients' basophils and ability to induce allergen-specific blocking IgG antibodies upon immunization.
resultsAlthough each of the mosaic proteins had lost the alpha-helical fold typical for Fel d 1, a strong reduction in IgE reactivity as well as allergenic activity in basophil activation assays was only obtained for three constructs, two reassembled fragments (Fel d 1 MB, Fel d 1 MC) and a fusion of the latter two (Fel d 1 MF) in which the cysteines of Fel d 1 MC were replaced by serines. Immunization of rabbits with Fel d 1 MB, MC and MF induced high levels of IgG antibodies that inhibited IgE reactivity of cat-allergic patients to Fel d 1 in a comparable manner as IgG induced with the wild-type allergen.
conclusionsWe report the development of hypoallergenic reassembled Fel d 1 proteins suitable for vaccination and tolerance induction in cat-allergic patients.
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