ArticleInternational journal of molecular and cellular medicine2014
A Multi-Locus Study for Detection of Cryptosporidium Species Isolated from Calves Population, Liverpool; UK.
Article in International journal of molecular and cellular medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Article
- Cryptosporidiosis: From Prevention to Treatment, a Narrative Review.Microorganisms · 2022Review
- Long-read assembly and comparative evidence-based reanalysis ofGenome research · 2022Article
- Common occurrence of Cryptosporidium hominis in asymptomatic and symptomatic calves in France.PLoS neglected tropical diseases · 2018Article
- Prevalence and genotyping of Cryptosporidium spp. in farm animals in Egypt.The Journal of veterinary medical science · 2014Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cryptosporidium is an obligate intracellular protozoan parasite infecting a wide range of hosts. The current study investigated the genetic profile of Cryptosporidium species in calves in Liverpool, England. Fifty-two calve fecal samples were collected from a farm and initially screened by Auramine Phenol, modified Ziehl-Neelsen and ELISA. PCR analysis of 18S rRNA gene was carried out for the positive samples. Then, positive PCR samples were genotyped by an 18S rRNA- based PCR-RFLP, COWP - based PCR- RFLP; PCR of GP60 and HSP70 genes. Additionally, sequence analysis was carried out based on representative isolates of four loci. Cryptosporidium oocysts and antigens were detected in 34 out of 52 (65.4%) samples using screening techniques. Genotype analysis showed the presence of C. hominis and C. parvum in one and thirteen samples, respectively. Furthermore, subtypes of C. hominis Ib, C. parvum IIa; C. parvum subtype 2 were identified by GP60 and HSP70 sequences, respectively. These findings indicate the diversity of the molecular characteristics of Cryptosporidium species in calves' isolates. Moreover, referring to the literature; we report two new subtypes of C. parvum IIa and a rare case of C. hominis Ib in calves population.
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Identifiers
24551819PMC3927392What OpenQuestion holds
Registered trials
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