ArticleInternational journal of molecular and cellular medicine2013
Sarcocystis cruzi: First Molecular Identification from Cattle in Iran.
Article in International journal of molecular and cellular medicine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 14 citations in OpenAlex.
- Prevalence and molecular analysis ofVeterinary and animal science · 2025Article
- Detection and phylogenetic analysis ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Article
- First molecular characterization ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2018Article
- Prevalence of Muscular Sarcosporidiosis in Slaughtered Domestic Pigs in Perak, Peninsular Malaysia.Tropical life sciences research · 2017Article
- Molecular identification of macroscopic and microscopic cysts of sarcocystis in sheep in north khorasan province, iran.International journal of molecular and cellular medicine · 2014Article
- Demonstration of Sarcocystis-like Parasites Found in Peripheral Blood.International journal of molecular and cellular medicine · 2014Article
- The Infestation of Supplied Mincemeat toIranian journal of parasitologyArticle
- Molecular Analysis of Sarcocystis Spp. Isolated from Sheep (Ovis aries) in Babol Area, Mazandaran Province, Northern Iran.Iranian journal of parasitologyArticle
- RFLP Analysis of Fragments of theIranian journal of parasitologyArticle
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sarcocystis is a genus of cyst-forming parasites infecting both animals and human. This study aimed to isolate coccidian tissue cysts from muscle of infected animals by a simple method in addition to molecular identification of Sarcocystis cruzi from the samples. The samples were obtained from commercial source in Babol, Northern Iran. Five grams of calf muscle was cut into 2-3 pieces in 30 ml of phosphate-buffered saline containing 0.1% Tween 80 and homogenized with IKA T25, DIGITAL ULTRA-TURRAX. The homogenate was filtrated twice and used for microscopy examination and molecular analysis. Polymerase chain reaction (PCR) and partial sequence analysis of the 18S ribosomal gene were used to identify the Sarcocystis species. Giemsa stain of the filtrated calf muscle samples showed that the sample had ellipse to around tissue cysts contained crescent-shaped bodies. The PCR of the 18SrDNA yielded an 1100 bp DNA band on agarose gel and sequence analysis of the DNA confirmed the isolate as S. cruzi. The sequence was deposited in GenBank by Accession No.KC508514. This is the first molecular identification of an isolate of S. cruzi in Iran.
Indexed as
Identifiers
24551802PMC3920535W2109766771What OpenQuestion holds
Registered trials
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