ArticleJournal of clinical and diagnostic research : JCDR2013
Random Amplified Polymorphic DNA (RAPD) Typing of Multidrug Resistant Enterococcus faecium Urinary Isolates from a Tertiary Care Centre, Northern India.
Article in Journal of clinical and diagnostic research : JCDR, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed.
- Molecular Characterization ofArchives of Razi Institute · 2023Article
- Agarose Gel Electrophoresis-Based RAPD-PCR-An Optimization of the Conditions to Rapidly Detect Similarity of the Alert Pathogens for the Purpose of Epidemiological Studies.Gels (Basel, Switzerland) · 2022Article
- Molecular characterization and antimicrobial resistance of Enterococcus faecalis isolated from seafood samples.Veterinary medicine and science · 2022Article
- Antibiotic Resistance And Genotyping Of Gram-Positive Bacteria Causing Hospital-Acquired Infection In Patients Referring To Children's Medical Center.Infection and drug resistance · 2019Article
- Antimicrobial resistance and virulence factor gene profiles of Enterococcus spp. isolates from wild Arctocephalus australis (South American fur seal) and Arctocephalus tropicalis (Subantarctic fur seal).World journal of microbiology & biotechnology · 2015Article
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Abstract
backgroundEnterococci, though they are a part of commensal flora, are becoming increasingly important as nosocomial pathogens, due to their inherited and acquired resistances to several antimicrobial agents. In this context, Enterococcus faecium (E.faecium) requires a special mention due to its characteristic of Multidrug Resistance (MDR) and its ability to disseminate.
aimThis study was undertaken to phenotypically characterize and determine clonal relatedness amongst the indoor isolates of Enterococcus faecium (E. faecium) which were isolated from patients with urinary tract infections (UTIs). SETTINGS AND
designThis study was carried out prospectively in a tertiary care university hospital and in Department of Microbiology, Varanasi, India. MATERIAL AND
methodsUrine samples were collected from patients who were admitted in different departments of the hospital with a clinical diagnosis of UTIs and they were processed for a period of one year. Enterococcal species were identified by doing extensive biochemical tests. Anti-microbial susceptibility testing was done by disc diffusion and agar dilution methods. Molecular typing of the isolates was done by Random Amplified Polymorphic DNA (RAPD) typing method.
resultsA total of 48 Enterococcal urinary isolates were identified in indoor patients, among which a majority (46, 95.83%) were E.faecium isolates. These isolates exhibited high resistance to fluoroquinolones (91.3%) and to ampicillin (60.86%) in particular. Two isolates were found to be resistant to vancomycin on screen agar. RAPD typing showed two major clusters, one of which had ten strains of 100% similarity, all of which were isolated from a common source.
conclusionThis study showed dissemination of multidrug resistant E. faecium isolates within the hospital. Being a quick and cost effective method, RAPD typing can be used to show clonal relatedness and to trace possible sources of organisms for epidemiological purposes.
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