ArticleInternational journal of clinical and experimental pathology2014
Apoptotic genes expression in placenta of clubfoot-like fetus pregnant rats.
Article in International journal of clinical and experimental pathology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed, 6 citations in OpenAlex.
- Histological changes in placental rat apoptosis via FasL and cytochromeSaudi journal of biological sciences · 2021Article
- Vitamin A Requirements in Pregnancy and Lactation.Current developments in nutrition · 2020Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo investigate the apoptotic gene expression of placenta in an all-trans-retinoic acid (ATRA) induced fetus congenital clubfoot pregnant rat model.
methodsSprague-Dawley (SD) rats were divided randomly into ATRA-exposed group and control group. On day 10 of pregnancy, a dose of 120 mg/kg ATRA dissolved in mineral oil was given intragastrically to the rats in the ATRA-exposed group and equivalent volume of mineral oil was given intragastrically to the control rats. Fetuses were delivered on day 20 of pregnancy, the placenta was collected for the pathological and biochemical analysis.
resultsClubfoot-like deformity fetuses were observed in the ATRA-exposed group and none with deformity was found in the control group. The pro-apoptosis in placenta of ATRA-exposed group was measured by flow cytometry. Moreover, compared with the control group, lower expression of Bcl-2 and higher expression of BAX were found in the ATRA-exposed group in both mRNA and protein level. Immunohistochemical labeling of Bcl-2 in the control group was more intense while BAX labeling in the ATRA-exposed group was more intense. Additionally, the caspase-3 activity was also significantly increased in the ATRA-exposed group than control group.
conclusionIn our research, we found a pro-apoptosis in placenta in the ATRA-exposed pregnant rats, indicating a possible association between placental apoptosis and congenital clubfoot.
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Identifiers
24551289PMC3925913W287083588What OpenQuestion holds
Registered trials
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