ArticlePLoS neglected tropical diseases2014
Molecular, immunological, and biological characterization of Tityus serrulatus venom hyaluronidase: new insights into its role in envenomation.
Article in PLoS neglected tropical diseases, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 61 citations in OpenAlex.
- Venom gland transcriptomics and bioactivity profiling suggest bifunctional hyaluronidase activity in the venom ofFrontiers in molecular biosciences · 2026Article
- Hyaluronidase treatment is associated with increased cytokine levels and reduced peritoneal neutrophil accumulation during LPS-induced inflammation.Frontiers in pharmacology · 2026Article
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- An overview of some enzymes from buthid scorpion venoms from Colombia:The journal of venomous animals and toxins including tropical diseases · 2024Article
- Understanding the complexity ofThe journal of venomous animals and toxins including tropical diseases · 2024Review
- Toxicity Mechanism of Dangerous Scorpion Stings in Iran.Journal of arthropod-borne diseases · 2023Review
- Phenotypic and Transcriptomics Analyses Reveal Underlying Mechanisms in a Mouse Model of Corneal Bee Sting.Toxins · 2022Article
- Overview of protein posttranslational modifications in Arthropoda venoms.The journal of venomous animals and toxins including tropical diseases · 2022Review
- Comparison of the Scorpionism Caused byToxins · 2021Article
- Scorpion species of medical importance in the Brazilian Amazon: a review to identify knowledge gaps.The journal of venomous animals and toxins including tropical diseases · 2021Article
- Prospective Use of Brown Spider Venom Toxins as Therapeutic and Biotechnological Inputs.Frontiers in molecular biosciences · 2021Review
- Inhibition of Tityus serrulatus venom hyaluronidase affects venom biodistribution.PLoS neglected tropical diseases · 2019Article
- Bee Updated: Current Knowledge on Bee Venom and Bee Envenoming Therapy.Frontiers in immunology · 2019Review
- CPP-Ts: a new intracellular calcium channel modulator and a promising tool for drug delivery in cancer cells.Scientific reports · 2018Article
- Antibody Cross-Reactivity in Antivenom Research.Toxins · 2018Review
- Transcriptomic and Proteomic Analyses Reveal the Diversity of Venom Components from the Vaejovid ScorpionToxins · 2018Article
- Article
- Proteomic endorsed transcriptomic profiles of venom glands from Tityus obscurus and T. serrulatus scorpions.PloS one · 2018Article
- Recombinant Protein Containing B-Cell Epitopes of DifferentFrontiers in immunology · 2018Article
- Tityus serrulatus Scorpion Venom: In Vitro Tests and Their Correlation with In Vivo Lethal Dose Assay.Toxins · 2017Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundScorpionism is a public health problem in Brazil, and Tityus serrulatus (Ts) is primarily responsible for severe accidents. The main toxic components of Ts venom are low-molecular-weight neurotoxins; however, the venom also contains poorly characterized high-molecular-weight enzymes. Hyaluronidase is one such enzyme that has been poorly characterized. METHODS AND PRINCIPAL
findingsWe examined clones from a cDNA library of the Ts venom gland and described two novel isoforms of hyaluronidase, TsHyal-1 and TsHyal-2. The isoforms are 83% identical, and alignment of their predicted amino acid sequences with other hyaluronidases showed conserved residues between evolutionarily distant organisms. We performed gel filtration followed by reversed-phase chromatography to purify native hyaluronidase from Ts venom. Purified native Ts hyaluronidase was used to produce anti-hyaluronidase serum in rabbits. As little as 0.94 µl of anti-hyaluronidase serum neutralized 1 LD50 (13.2 µg) of Ts venom hyaluronidase activity in vitro. In vivo neutralization assays showed that 121.6 µl of anti-hyaluronidase serum inhibited mouse death 100%, whereas 60.8 µl and 15.2 µl of serum delayed mouse death. Inhibition of death was also achieved by using the hyaluronidase pharmacological inhibitor aristolochic acid. Addition of native Ts hyaluronidase (0.418 µg) to pre-neutralized Ts venom (13.2 µg venom+0.94 µl anti-hyaluronidase serum) reversed mouse survival. We used the SPOT method to map TsHyal-1 and TsHyal-2 epitopes. More peptides were recognized by anti-hyaluronidase serum in TsHyal-1 than in TsHyal-2. Epitopes common to both isoforms included active site residues.
conclusionsHyaluronidase inhibition and immunoneutralization reduced the toxic effects of Ts venom. Our results have implications in scorpionism therapy and challenge the notion that only neurotoxins are important to the envenoming process.
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