ArticlePloS one2014
Single cell genomics of uncultured, health-associated Tannerella BU063 (Oral Taxon 286) and comparison to the closely related pathogen Tannerella forsythia.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed.
- Metagenome and Metabolic Pathways in Plaque Biofilms of Thai ELANE-Associated Neutropenic Patients: An Original Study and Scoping Review.European journal of dentistry · 2026Article
- Exploring the oral microbiome: from traditional techniques to advanced omics and databases.Antonie van Leeuwenhoek · 2026Review
- Metagenomic Assessment of Full-Scale Wastewater Treatment Plants Identifies Sentinel Antibiotic Resistance Gene Families for Monitoring Reclaimed Wastewater and Treated Sludge.Environmental science & technology · 2026Article
- The metagenome and metabolome signatures of dental biofilms associated with severe dental fluorosis.Journal of oral microbiology · 2025Article
- Illuminating the oral microbiome and its host interactions: recent advancements in omics and bioinformatics technologies in the context of oral microbiome research.FEMS microbiology reviews · 2023Review
- Microbiota in Periodontitis: Advances in the Omic Era.Advances in experimental medicine and biology · 2022Review
- Potential role for age as a modulator of oral nitrate reductase activity.Nitric oxide : biology and chemistry · 2021Article
- Single-Cell Genomics and the Oral Microbiome.Journal of dental research · 2020Review
- Persistence ofCurrent oral health reports · 2020Article
- A completeness-independent method for pre-selection of closely related genomes for species delineation in prokaryotes.BMC genomics · 2020Article
- Comparative genome characterization of the periodontal pathogen Tannerella forsythia.BMC genomics · 2020Article
- Metabolic network percolation quantifies biosynthetic capabilities across the human oral microbiome.eLife · 2019Article
- Nonulosonic acids contribute to the pathogenicity of the oral bacteriumInterface focus · 2019Review
- A General ProteinFrontiers in microbiology · 2018Article
- The Host Microbiome Regulates and Maintains Human Health: A Primer and Perspective for Non-Microbiologists.Cancer research · 2017Review
- SAG-QC: quality control of single amplified genome information by subtracting non-target sequences based on sequence compositions.BMC bioinformatics · 2017Article
- Oral microbiome and peri-implant diseases: where are we now?Therapeutics and clinical risk management · 2017Review
- First Cultivation of Health-Associated Tannerella sp. HOT-286 (BU063).Journal of dental research · 2016Article
- Culture Independent Genomic Comparisons Reveal Environmental Adaptations for Altiarchaeales.Frontiers in microbiology · 2016Article
- Inositol-phosphodihydroceramides in the periodontal pathogen Tannerella forsythia: Structural analysis and incorporation of exogenous myo-inositol.Biochimica et biophysica acta · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
The uncultivated bacterium Tannerella BU063 (oral taxon 286) is the closest relative to the periodontal pathogen Tannerella forsythia, but is not disease-associated itself. Using a single cell genomics approach, we isolated 12 individual BU063 cells by flow cytometry, and we amplified and sequenced their genomes. Comparative analyses of the assembled genomic scaffolds and their gene contents allowed us to study the diversity of this taxon within the oral community of a single human donor that provided the sample. Eight different BU063 genotypes were represented, all about 5% divergent at the nucleotide level. There were 2 pairs of cells and one group of three that were more highly identical, and may represent clonal populations. We did pooled assemblies on the nearly identical genomes to increase the assembled genomic coverage. The presence of a set of 66 "core" housekeeping genes showed that two of the single cell assemblies and the assembly derived from the three putatively identical cells were essentially complete. As expected, the genome of BU063 is more similar to Tannerella forsythia than any other known genome, although there are significant differences, including a 44% difference in gene content, changes in metabolic pathways, loss of synteny, and an 8-9% difference in GC content. Several identified virulence genes of T. forsythia are not found in BU063 including karilysin, prtH, and bspA. The absence of these genes may explain the lack of periodontal pathogenesis by this species and provides a new foundation to further understand the genome evolution and mechanisms of bacterial-host interaction in closely related oral microbes with different pathogenicity potential.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.