ArticlePloS one2014
Identification and functional characterization of two executioner caspases in Crassostrea gigas.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 76 citations in OpenAlex.
- Protective Effects of Lithium Borate on Acute Lung Injury Induced by Intestinal Ischemia/Reperfusion in Rats.ACS omega · 2026Article
- Sublethal exposure of eastern oyster Crassostrea virginica to the goniodomin-producing dinoflagellate Alexandrium monilatum: Fate of toxins, histopathology, and gene expression.Journal of aquatic animal health · 2024Article
- PI3K-AKT-mediated phosphorylation of Thr260 in CgCaspase-3/6/7 regulates heat-induced activation in oysters.Communications biology · 2024Article
- The Caspase Homologues in ScallopToxins · 2022Article
- Phylogenetic analysis of the caspase family in bivalves: implications for programmed cell death, immune response and development.BMC genomics · 2021Article
- The glutaminase (CgGLS-1) mediates anti-bacterial immunity by prompting cytokine synthesis and hemocyte apoptosis in Pacific oyster Crassostrea gigas.Scientific reports · 2021Article
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- Functional miR-142a-3p Induces Apoptosis and Macrophage Polarization by TargetingFrontiers in immunology · 2021Article
- Bivalves are NO different: nitric oxide as negative regulator of metamorphosis in the Pacific oyster, Crassostrea gigas.BMC developmental biology · 2020Article
- Caspase dependent apoptosis is required for anterior regeneration in Aeolosoma viride and its related gene expressions are regulated by the Wnt signaling pathway.Scientific reports · 2020Article
- Gene expression profiles at different stages for formation of pearl sac and pearl in the pearl oyster Pinctada fucata.BMC genomics · 2019Article
- Starfish Apaf-1 activates effector caspase-3/9 upon apoptosis of aged eggs.Scientific reports · 2018Article
- Conservation and divergence of mitochondrial apoptosis pathway in the Pacific oyster, Crassostrea gigas.Cell death & disease · 2017Article
- Intestinal ischemic preconditioning reduces liver ischemia reperfusion injury in rats.Molecular medicine reports · 2016Article
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- Molecular cloning of two molluscan caspases and gene functional analysis during Crassostrea angulata (Fujian oyster) larval metamorphosis.Molecular biology reports · 2015Article
- Protective effect of intestinal ischemic preconditioning on ischemia reperfusion-caused lung injury in rats.Inflammation · 2015Article
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Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Caspase-3 and caspase-7 are two key effector caspases that play important roles in apoptotic pathways that maintain normal tissue and organ development and homeostasis. However, little is known about the sequence, structure, activity, and function of effector caspases upon apoptosis in mollusks, especially marine bivalves. In this study, we investigated the possible roles of two executioner caspases in the regulation of apoptosis in the Pacific oyster Crassostrea gigas. A full-length caspase-3-like gene named Cgcaspase-3 was cloned from C.gigas cDNA, encoding a predicted protein containing caspase family p20 and p10 domain profiles and a conserved caspase active site motif. Phylogenetic analysis demonstrated that both Cgcaspase-3 and Cgcaspase-1 may function as effector caspases clustered in the invertebrate branch. Although the sequence identities between the two caspases was low, both enzymes possessed executioner caspase activity and were capable of inducing cell death. These results suggested that Cgcaspase-3 and Cgcaspase-1 were two effector caspases in C. gigas. We also observed that nucleus-localized Cgcaspase-3, may function as a caspase-3-like protein and cytoplasm-localized Cgcaspase-1 may function as a caspase-7-like protein. Both Cgcaspase-3 and Cgcaspase-1 mRNA expression increased after larvae settled on the substratum, suggesting that both caspases acted in several tissues or organs that degenerated after oyster larvae settlement. The highest caspase expression levels were observed in the gills indicating that both effector caspases were likely involved in immune or metabolic processes in C. gigas.
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