ArticlePloS one2014
Detection of live circulating tumor cells by a class of near-infrared heptamethine carbocyanine dyes in patients with localized and metastatic prostate cancer.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Specific targeting of cancer cell mitochondria with a malaria drug improves pancreatic cancer outcome.Scientific reports · 2026Article
- Combined mass spectrometry and computational analysis of cyanine dyes and their structural analogues.RSC advances · 2026Article
- Overcoming Resistance in Prostate Cancer Therapy Using a DZ-Simvastatin Conjugate.Molecular pharmaceutics · 2024Article
- Article
- A cisplatin conjugate with tumor cell specificity exhibits antitumor effects in renal cancer models.BMC cancer · 2023Article
- Accurate prostate cancer detection based on enrichment and characterization of prostate cancer specific circulating tumor cells.Cancer medicine · 2023Article
- Deep learning assisted holography microscopy for in-flow enumeration of tumor cells in blood.RSC advances · 2023Article
- Novel DZ-SIM Conjugate Targets Cancer Mitochondria and Prolongs Survival in Pancreatic Ductal Adenocarcinoma.Advanced therapeutics · 2022Article
- Detection of live breast cancer cells in bright-field microscopy images containing white blood cells by image analysis and deep learning.Journal of biomedical optics · 2022Article
- Predictive Value of Circulating Tumor Cells Detected by ISETLife (Basel, Switzerland) · 2022Article
- Circulating Fatty Objects and Their Preferential Presence in Pancreatic Cancer Patient Blood Samples.Frontiers in physiology · 2022Article
- A heptamethine cyanine dye serves as a potential marker for myeloid-derived suppressor cells.American journal of cancer research · 2021Article
- Development of small molecule fluorescent dye drug conjugates in targeted cancer therapy.American journal of clinical and experimental urology · 2021Article
- The Use of Heptamethine Cyanine Dyes as Drug-Conjugate Systems in the Treatment of Primary and Metastatic Brain Tumors.Frontiers in oncology · 2021Review
- Polymethine Dye-Functionalized Nanoparticles for Targeting CML Stem Cells.Molecular therapy oncolytics · 2020Article
- Nanotechnology-Assisted Isolation and Analysis of Circulating Tumor Cells on Microfluidic Devices.Micromachines · 2020Review
- Application of electrochemical biosensors in tumor cell detection.Thoracic cancer · 2020Review
- Comprehensive Effects of Near-Infrared Multifunctional Liposomes on Cancer Cells.Molecules (Basel, Switzerland) · 2020Article
- IR-783 inhibits breast cancer cell proliferation and migration by inducing mitochondrial fission.International journal of oncology · 2019Article
- Review
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15 authors.
Funding
Abstract
Tumor cells are inherently heterogeneous and often exhibit diminished adhesion, resulting in the shedding of tumor cells into the circulation to form circulating tumor cells (CTCs). A fraction of these are live CTCs with potential of metastatic colonization whereas others are at various stages of apoptosis making them likely to be less relevant to understanding the disease. Isolation and characterization of live CTCs may augment information yielded by standard enumeration to help physicians to more accurately establish diagnosis, choose therapy, monitor response, and provide prognosis. We previously reported on a group of near-infrared (NIR) heptamethine carbocyanine dyes that are specifically and actively transported into live cancer cells. In this study, this viable tumor cell-specific behavior was utilized to detect live CTCs in prostate cancer patients. Peripheral blood mononuclear cells (PBMCs) from 40 patients with localized prostate cancer together with 5 patients with metastatic disease were stained with IR-783, the prototype heptamethine cyanine dye. Stained cells were subjected to flow cytometric analysis to identify live (NIR(+)) CTCs from the pool of total CTCs, which were identified by EpCAM staining. In patients with localized tumor, live CTC counts corresponded with total CTC numbers. Higher live CTC counts were seen in patients with larger tumors and those with more aggressive pathologic features including positive margins and/or lymph node invasion. Even higher CTC numbers (live and total) were detected in patients with metastatic disease. Live CTC counts declined when patients were receiving effective treatments, and conversely the counts tended to rise at the time of disease progression. Our study demonstrates the feasibility of applying of this staining technique to identify live CTCs, creating an opportunity for further molecular interrogation of a more biologically relevant CTC population.
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