Evidence map›Paper›PMID 24551031›Full record

ArticlePloS one2014

Identifying microRNAs and transcript targets in Jatropha seeds.

Vanessa Galli, Frank Guzman, Luiz F V de Oliveira, Guilherme Loss-Morais, Ana P Körbes, Sérgio D A Silva, Márcia M A N Margis-Pinheiro, Rogério Margis

Open access · goldAbstract read
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
8.9field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 64 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. MicroRNAs in Woody Plants.Frontiers in plant science · 2021
    Review
  5. Article
  6. Article
  7. Article
  8. Article
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  10. Article
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  15. Article
  16. Review
  17. Article
  18. The role of microRNAs in gallbladder cancer.Molecular and clinical oncology · 2016
    Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Vanessa GalliCenter of Biotechnology and PPGBCM, Laboratory of Genomes and Plant Populations, Federal University of Rio Grande do Sul - UFRGS, Porto Alegre, RS, Brazil ; Brazilian Agricultural Research - EMBRAPA, Pelotas, RS, Brazil.
Frank GuzmanPPGGBM at Federal University of Rio Grande do Sul - UFRGS, Porto Alegre, RS, Brazil.
Luiz F V de OliveiraCenter of Biotechnology and PPGBCM, Laboratory of Genomes and Plant Populations, Federal University of Rio Grande do Sul - UFRGS, Porto Alegre, RS, Brazil.
Guilherme Loss-MoraisCenter of Biotechnology and PPGBCM, Laboratory of Genomes and Plant Populations, Federal University of Rio Grande do Sul - UFRGS, Porto Alegre, RS, Brazil.
Ana P KörbesPPGGBM at Federal University of Rio Grande do Sul - UFRGS, Porto Alegre, RS, Brazil.
Sérgio D A SilvaBrazilian Agricultural Research - EMBRAPA, Pelotas, RS, Brazil.
Márcia M A N Margis-PinheiroPPGGBM at Federal University of Rio Grande do Sul - UFRGS, Porto Alegre, RS, Brazil.
Rogério MargisCenter of Biotechnology and PPGBCM, Laboratory of Genomes and Plant Populations, Federal University of Rio Grande do Sul - UFRGS, Porto Alegre, RS, Brazil ; PPGGBM at Federal University of Rio Grande do Sul - UFRGS, Porto Alegre, RS, Brazil.
Universidade Federal do Rio Grande do Sul · BRBrazilian Agricultural Research Corporation · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

MicroRNAs, or miRNAs, are endogenously encoded small RNAs that play a key role in diverse plant biological processes. Jatropha curcas L. has received significant attention as a potential oilseed crop for the production of renewable oil. Here, a sRNA library of mature seeds and three mRNA libraries from three different seed development stages were generated by deep sequencing to identify and characterize the miRNAs and pre-miRNAs of J. curcas. Computational analysis was used for the identification of 180 conserved miRNAs and 41 precursors (pre-miRNAs) as well as 16 novel pre-miRNAs. The predicted miRNA target genes are involved in a broad range of physiological functions, including cellular structure, nuclear function, translation, transport, hormone synthesis, defense, and lipid metabolism. Some pre-miRNA and miRNA targets vary in abundance between the three stages of seed development. A search for sequences that produce siRNA was performed, and the results indicated that J. curcas siRNAs play a role in nuclear functions, transport, catalytic processes and disease resistance. This study presents the first large scale identification of J. curcas miRNAs and their targets in mature seeds based on deep sequencing, and it contributes to a functional understanding of these miRNAs.

Indexed as

Gene Expression Regulation, PlantGene Expression Regulation, DevelopmentalGene LibraryHigh-Throughput Nucleotide SequencingJatrophaMetabolic Networks and PathwaysMicroRNAsMolecular Sequence AnnotationRNA, MessengerRNA, PlantSeedsMicroRNAsRNA, MessengerRNA, Plant

Identifiers

PMID24551031
PMCPMC3923737
OpenAlexW2032942014

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.