Evidence map›Paper›PMID 24550372›Full record

ArticleGut2014

The stem cell organisation, and the proliferative and gene expression profile of Barrett's epithelium, replicates pyloric-type gastric glands.

Danielle L Lavery, Anna M Nicholson, Richard Poulsom, Rosemary Jeffery, Alia Hussain, Laura J Gay, Janusz A Jankowski, Sebastian S Zeki, Hugh Barr, Rebecca Harrison and 10 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Gut, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.

0numbers the graph read from it
0cells of the map it votes in
51citing papers in PubMed
7.6field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

51 citing papers in PubMed, 81 citations in OpenAlex.

  1. Randomized Controlled Trial of the Gastrin/CCKCancer prevention research (Philadelphia, Pa.) · 2021
    Trial
  2. Review
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  4. Article
  5. Article
  6. Review
  7. Article
  8. Review
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  10. Cellular Origins and Lineage Plasticity in Cancer.Cold Spring Harbor perspectives in medicine · 2024
    Review
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  12. Review
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  14. Article
  15. Review
  16. Article
  17. Article
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

20 authors at 10 institutions in 2 countries.

Danielle L LaveryEpithelial Stem Cell Group, Centre for Tumour Biology, Barts Cancer Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
Anna M NicholsonStem Cell Biology of the Intestine Laboratory, Cancer Research UK Cambridge Research Institute, Li Ka Shing Centre, Cambridge, UK.
Richard PoulsomCentre for Digestive Diseases, Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK.ORCID http://orcid.org/0000-0001-8567-3543
Rosemary JefferyCentre for Digestive Diseases, Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
Alia HussainEpithelial Stem Cell Group, Centre for Tumour Biology, Barts Cancer Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
Laura J GayEpithelial Stem Cell Group, Centre for Tumour Biology, Barts Cancer Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
Janusz A JankowskiCentre for Digestive Diseases, Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
Sebastian S ZekiEpithelial Stem Cell Group, Centre for Tumour Biology, Barts Cancer Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
Hugh BarrDepartment of Surgery, Gloucestershire Royal Hospital, Gloucestershire Royal Hospital, Gloucester, UK.
Rebecca HarrisonDepartment of Pathology, University Hospitals Leicester, Leicester, UK.
James GoingUniversity of Glasgow, Institute of Cancer Sciences, Glasgow, UK.
Sritharan KadirkamanathanMid Essex Hospital Services NHS Trust, Broomfield Hospital, Chelmsford, UK.
Peter DavisMid Essex Hospital Services NHS Trust, Broomfield Hospital, Chelmsford, UK.
Timothy UnderwoodFaculty of Medicine, University of Southampton, Southampton, UK.
Marco R NovelliDepartment of Histopathology, University College London, London, UK.
Manuel Rodriguez-JustoDepartment of Histopathology, University College London, London, UK.
Neil ShepherdGloucestershire Cellular Pathology Laboratory, Cheltenham General Hospital, Cheltenham, UK.
Marnix JansenDepartment of Pathology, Academic Medical Center (AMC), Amsterdam, The Netherlands.
Nicholas A WrightEpithelial Stem Cell Group, Centre for Tumour Biology, Barts Cancer Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
Stuart A C McDonaldEpithelial Stem Cell Group, Centre for Tumour Biology, Barts Cancer Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
Queen Mary University of London · GBBroomfield Hospital · GBUniversity College London · GBAmsterdam UMC Location University of Amsterdam · NLCancer Research UK · GBCheltenham General Hospital · GBGloucestershire Royal Hospital · GBUniversity Hospitals of Leicester NHS Trust · GBUniversity of Glasgow · GBUniversity of Southampton · GB

Funding

Cancer Research UK 14895Cancer Research UK 4584Medical Research Council G1002565
6 · The paper itself

Abstract

objectiveBarrett's oesophagus shows appearances described as 'intestinal metaplasia', in structures called 'crypts' but do not typically display crypt architecture. Here, we investigate their relationship to gastric glands.

methodsCell proliferation and migration within Barrett's glands was assessed by Ki67 and iododeoxyuridine (IdU) labelling. Expression of mucin core proteins (MUC), trefoil family factor (TFF) peptides and LGR5 mRNA was determined by immunohistochemistry or by in situ hybridisation, and clonality was elucidated using mitochondrial DNA (mtDNA) mutations combined with mucin histochemistry.

resultsProliferation predominantly occurs in the middle of Barrett's glands, diminishing towards the surface and the base: IdU dynamics demonstrate bidirectional migration, similar to gastric glands. Distribution of MUC5AC, TFF1, MUC6 and TFF2 in Barrett's mirrors pyloric glands and is preserved in Barrett's dysplasia. MUC2-positive goblet cells are localised above the neck in Barrett's glands, and TFF3 is concentrated in the same region. LGR5 mRNA is detected in the middle of Barrett's glands suggesting a stem cell niche in this locale, similar to that in the gastric pylorus, and distinct from gastric intestinal metaplasia. Gastric and intestinal cell lineages within Barrett's glands are clonal, indicating derivation from a single stem cell.

conclusionsBarrett's shows the proliferative and stem cell architecture, and pattern of gene expression of pyloric gastric glands, maintained by stem cells showing gastric and intestinal differentiation: neutral drift may suggest that intestinal differentiation advances with time, a concept critical for the understanding of the origin and development of Barrett's oesophagus.

Indexed as

Barrett EsophagusEsophagusBiomarkers, TumorCell MovementCell ProliferationDisease ProgressionGastric MucosaGene Expression ProfilingGoblet CellsHumansIdoxuridineImmunohistochemistryKi-67 AntigenMucin 5ACNucleic Acid Synthesis InhibitorsPeptidesBiomarkers, TumorIdoxuridineKi-67 AntigenLGR5 protein, humanMUC5AC protein, humanMucin 5ACNucleic Acid Synthesis InhibitorsPeptidesReceptors, G-Protein-CoupledTFF2 protein, humanTFF3 protein, humanTrefoil Factor-2Trefoil Factor-3Barrett's OesophagusGene ExpressionMucinsStem CellsTrefoil Factors

Identifiers

PMID24550372
PMCPMC4251192
OpenAlexW2055561239

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.