ArticleInvestigative ophthalmology & visual science2014
Vascular endothelial growth factor modulates the function of the retinal pigment epithelium in vivo.
Article in Investigative ophthalmology & visual science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 24 citations in OpenAlex.
- Photoreceptor cells and RPE contribute to the development of diabetic retinopathy.Progress in retinal and eye research · 2021Review
- Subtle changes in diabetic retinas localised in 3D using OCT.Ophthalmic & physiological optics : the journal of the British College of Ophthalmic Opticians (Optometrists) · 2018Article
- A2E-associated cell death and inflammation in retinal pigmented epithelial cells from human induced pluripotent stem cells.Stem cell research · 2018Article
- Targeting the tight junction protein, zonula occludens-1, with the connexin43 mimetic peptide, αCT1, reduces VEGF-dependent RPE pathophysiology.Journal of molecular medicine (Berlin, Germany) · 2017Article
- Comparison of Cysts in Red and Green Images for Diabetic Macular Edema.Optometry and vision science : official publication of the American Academy of Optometry · 2017Article
- Diabetic retinal pigment epitheliopathy: fundus autofluorescence and spectral-domain optical coherence tomography findings.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2016Article
- Progressive Early Breakdown of Retinal Pigment Epithelium Function in Hyperglycemic Rats.Investigative ophthalmology & visual science · 2016Article
- Histone Deacetylase Inhibition Restores Retinal Pigment Epithelium Function in Hyperglycemia.PloS one · 2016Article
- Receptor mediated disruption of retinal pigment epithelium function in acute glycated-albumin exposure.Experimental eye research · 2015Article
- The MAPK signaling pathway mediates the GPR91-dependent release of VEGF from RGC-5 cells.International journal of molecular medicine · 2015Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
purposeRetinal edema, the accumulation of extracellular fluid in the retina is usually attributed to inner blood retina barrier (BRB) leakage. Vascular endothelial growth factor plays an important role in this process. The effects of VEGF on the outer BRB, the RPE, however, have received limited attention. Here, we present a methodology to assess how VEGF modulates the integrity of the RPE barrier in vivo.
methodsControl subretinal blebs (1-5 μL) and blebs containing VEGF (1-100 μg/mL), placental growth factor (PlGF; 100 μg/mL), or albumin (100-1000 μg/mL) were injected into New Zealand White or Dutch Belted rabbits with IOP maintained at 10, 15, or 20 mm Hg. One-hour intravitreal pretreatment with ZM323881 (10 μM/L) was used to inhibit the VEGF response. Fluid resorption was followed by optical coherence tomography for 1 hour. Retinal pigment epithelium leakage was assessed by fluorescein angiography.
resultsIncreasing IOP resulted in an elevated rate of bleb resorption, while increasing albumin concentration in the bleb decreased the rate of resorption. Vascular endothelial growth factor, but not PlGF, caused a significant, concentration-dependent decrease in the rate of fluid resorption, which was reversed by ZM323881. Compared with albumin-filled blebs, VEGF-filled blebs showed accelerated early-phase leakage from the choroid.
conclusionsConsistent with a localized modulation of RPE function, VEGF induced a significant reduction in fluid resorption and an increase in hydraulic conductivity. Our results establish VEGF as a major cytokine regulating RPE barrier properties in vivo and indicate that the RPE is a principal factor in the pathogenesis of retinal edema.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.