ArticleProceedings of the National Academy of Sciences of the United States of America2014
Secretion of a pneumococcal type II secretion system pilus correlates with DNA uptake during transformation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 38 citations in OpenAlex.
- Article
- Universal receptive system as a novel regulator of transcriptomic activity of Staphylococcus aureus.Microbial cell factories · 2025Article
- Molecular mechanisms and applications of natural transformation in bacteria.Frontiers in microbiology · 2025Review
- The global proteome ofFrontiers in cellular and infection microbiology · 2025Article
- Article
- Mechanism of assembly of type 4 filaments: everything you always wanted to know (but were afraid to ask).Microbiology (Reading, England) · 2023Review
- A multiomics analysis of direct interkingdom dynamics between influenza A virus and Streptococcus pneumoniae uncovers host-independent changes to bacterial virulence fitness.PLoS pathogens · 2022Article
- Membrane particles evoke a serotype-independent cross-protection against pneumococcal infection that is dependent on the conserved lipoproteins MalX and PrsA.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- The dental plaque biofilm matrix.Periodontology 2000 · 2021Review
- Possible drugs for the treatment of bacterial infections in the future: anti-virulence drugs.The Journal of antibiotics · 2021Review
- The Role of Minor Pilins in Assembly and Function of the Competence Pilus ofFrontiers in cellular and infection microbiology · 2021Article
- Resistome ofACS infectious diseases · 2020Article
- Prophage-Mediated Disruption of Genetic Competence in Staphylococcus pseudintermedius.mSystems · 2020Article
- The Clustered Regularly Interspaced Short Palindromic Repeat System and Argonaute: An Emerging Bacterial Immunity System for Defense Against Natural Transformation?Frontiers in microbiology · 2020Review
- Article
- DNA Uptake by Type IV Filaments.Frontiers in molecular biosciences · 2019Review
- Article
- Structure of the competence pilus major pilin ComGC inThe Journal of biological chemistry · 2017Article
- Uptake of extracellular DNA: competence induced pili in natural transformation of Streptococcus pneumoniae.BioEssays : news and reviews in molecular, cellular and developmental biology · 2015Article
- Conserved Streptococcus pneumoniae spirosomes suggest a single type of transformation pilus in competence.PLoS pathogens · 2015Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Streptococcus pneumoniae is a major human pathogen that successfully adapts to the host environment via an efficient uptake system for free DNA liberated from other organisms in the upper respiratory tract, facilitating immune evasion and drug resistance. Although the initial signaling events leading to pneumococcal competence for DNA transformation and the fate of DNA when it has been taken up have been extensively studied, the actual mechanism by which DNA in the environment may traverse the thick capsular and cell wall layers remains unknown. Here we visualize that induction of competence results in the formation of a native morphologically distinct pilus structure on the bacterial surface. This plaited pilus is encoded by the competence (com)G locus, and, after assembly, it is rapidly released into the surrounding medium. Heterologous pneumococcal pilus expression in Escherichia coli was obtained by replacing the pulE-K putative pilin genes of the Klebsiella oxytoca type II secretion system with the complete comG locus. In the pneumococcus, the coordinated secretion of pili from the cells correlates to DNA transformation. A model for DNA transformation is proposed whereby pilus assembly "drills" a channel across the thick cell wall that becomes transiently open by secretion of the pilus, providing the entry port for exogenous DNA to gain access to DNA receptors associated with the cytoplasmic membrane.
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