ArticleProceedings of the National Academy of Sciences of the United States of America2014
Variation in MPK12 affects water use efficiency in Arabidopsis and reveals a pleiotropic link between guard cell size and ABA response.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
50 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Greater aperture counteracts effects of reduced stomatal density on water use efficiency: a case study on sugarcane and meta-analysis.Journal of experimental botany · 2024Pooled it
- Guard cell signal transduction mechanisms controlling stomatal movements by light, abscisic acid, CO2, VPD and heat.Plant physiology · 2026Review
- MYB59 is Linked to Natural Variation of Water-Use Associated With Warmer Temperatures inPlant-environment interactions (Hoboken, N.J.) · 2026Article
- Are PP2Cs with intrinsically disordered regions CO2 sensors that control stomatal movements?Plant physiology · 2026Article
- Stomatal CO2 sensing in plants: control of gas exchange and interactions with environmental stimuli.Plant & cell physiology · 2025Review
- Evolution of drought resistance strategies following the introduction of white clover (Trifolium repens L.).Annals of botany · 2025Article
- The genomics and physiology of abiotic stressors associated with global elevational gradients in Arabidopsis thaliana.The New phytologist · 2024Article
- Stomata: custodians of leaf gaseous exchange.Journal of experimental botany · 2024Article
- Reducing stomatal density by expression of a synthetic epidermal patterning factor increases leaf intrinsic water use efficiency and reduces plant water use in a C4 crop.Journal of experimental botany · 2024Article
- GABA does not regulate stomatal CO2 signalling in Arabidopsis.Journal of experimental botany · 2024Article
- Stomatal development in the changing climate.Development (Cambridge, England) · 2024Review
- Natural variation in response to combined water and nitrogen deficiencies in Arabidopsis.The Plant cell · 2024Article
- Abiotic Stress Tolerance Boosted by Genetic Diversity in Plants.International journal of molecular sciences · 2024Article
- A large-effect fitness trade-off across environments is explained by a single mutation affecting cold acclimation.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Dynamic QTL mapping revealed primarily the genetic structure of photosynthetic traits in castor (Ricinus communis L.).Scientific reports · 2023Article
- Review
- MPK12 in stomatal COThe New phytologist · 2023Article
- Genomic Basis of Adaptation to a Novel Precipitation Regime.Molecular biology and evolution · 2023Article
- Burning questions for a warming and changing world: 15 unknowns in plant abiotic stress.The Plant cell · 2023Article
- Stomatal COScience advances · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant water relations are critical for determining the distribution, persistence, and fitness of plant species. Studying the genetic basis of ecologically relevant traits, however, can be complicated by their complex genetic, physiological, and developmental basis and their interaction with the environment. Water use efficiency (WUE), the ratio of photosynthetic carbon assimilation to stomatal conductance to water, is a dynamic trait with tremendous ecological and agricultural importance whose genetic control is poorly understood. In the present study, we use a quantitative trait locus-mapping approach to locate, fine-map, clone, confirm, and characterize an allelic substitution that drives differences in WUE among natural accessions of Arabidopsis thaliana. We show that a single amino acid substitution in an abscisic acid-responsive kinase, AtMPK12, causes reduction in WUE, and we confirm its functional role using transgenics. We further demonstrate that natural alleles at AtMPK12 differ in their response to cellular and environmental cues, with the allele from the Cape Verde Islands (CVI) being less responsive to hormonal inhibition of stomatal opening and more responsive to short-term changes in vapor pressure deficit. We also show that the CVI allele results in constitutively larger stomata. Together, these differences cause higher stomatal conductance and lower WUE compared with the common allele. These physiological changes resulted in reduced whole-plant transpiration efficiency and reduced fitness under water-limited compared with well-watered conditions. Our work demonstrates how detailed analysis of naturally segregating functional variation can uncover the molecular and physiological basis of a key trait associated with plant performance in ecological and agricultural settings.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.