ArticleProceedings of the National Academy of Sciences of the United States of America2014
SAC phosphoinositide phosphatases at the tonoplast mediate vacuolar function in Arabidopsis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 72 citations in OpenAlex.
- Regulation of shoot gravitropism and branching angle by the GRV2-SAC1 axis in Arabidopsis.The Plant journal : for cell and molecular biology · 2026Article
- Barley resistance and susceptibility to fungal cell entry involve the interplay of ROP signaling with phosphatidylinositol-monophosphates.The Plant journal : for cell and molecular biology · 2025Article
- TheeLife · 2022Article
- Phosphatidylinositol 3-phosphate regulates SCAB1-mediated F-actin reorganization during stomatal closure in Arabidopsis.The Plant cell · 2022Article
- Inhibition of Very Long Chain Fatty Acids Synthesis Mediates PI3P Homeostasis at Endosomal Compartments.International journal of molecular sciences · 2021Article
- Functions and Mechanisms of SAC Phosphoinositide Phosphatases in Plants.Frontiers in plant science · 2021Review
- Tonoplast-localized CaProceedings of the National Academy of Sciences of the United States of America · 2020Article
- A SAC Phosphoinositide Phosphatase Controls Rice Development via Hydrolyzing PI4P and PI(4,5)PPlant physiology · 2020Article
- LPIAT, aInternational journal of molecular sciences · 2020Article
- Stimulation of adventitious root formation by the oligosaccharin OSRG at the transcriptome level.Plant signaling & behavior · 2020Article
- DownregulatingFrontiers in plant science · 2020Article
- The Function of Inositol Phosphatases in Plant Tolerance to Abiotic Stress.International journal of molecular sciences · 2019Review
- Polar vacuolar distribution is essential for accurate asymmetric division ofProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Anionic Lipids: A Pipeline Connecting Key Players of Plant Cell Division.Frontiers in plant science · 2019Review
- Diverse Physiological Functions of FAB1 and Phosphatidylinositol 3,5-Bisphosphate in Plants.Frontiers in plant science · 2019Review
- Phosphoinositides control the localization of HOPS subunit VPS41, which together with VPS33 mediates vacuole fusion in plants.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- ArabidopsisPlant physiology · 2018Article
- Brassinosteroids regulate vacuolar morphology in root meristem cells of Arabidopsis thaliana.Plant signaling & behavior · 2018Article
- Vacuolar Trafficking Protein VPS38 Is Dispensable for Autophagy.Plant physiology · 2018Article
- The signaling lipid phosphatidylinositol-3,5-bisphosphate targets plant CLC-a anion/HEMBO reports · 2017Article
Corrections and comments
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Authors and funding
15 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phosphatidylinositol (PtdIns) is a structural phospholipid that can be phosphorylated into various lipid signaling molecules, designated polyphosphoinositides (PPIs). The reversible phosphorylation of PPIs on the 3, 4, or 5 position of inositol is performed by a set of organelle-specific kinases and phosphatases, and the characteristic head groups make these molecules ideal for regulating biological processes in time and space. In yeast and mammals, PtdIns3P and PtdIns(3,5)P2 play crucial roles in trafficking toward the lytic compartments, whereas the role in plants is not yet fully understood. Here we identified the role of a land plant-specific subgroup of PPI phosphatases, the suppressor of actin 2 (SAC2) to SAC5, during vacuolar trafficking and morphogenesis in Arabidopsis thaliana. SAC2-SAC5 localize to the tonoplast along with PtdIns3P, the presumable product of their activity. In SAC gain- and loss-of-function mutants, the levels of PtdIns monophosphates and bisphosphates were changed, with opposite effects on the morphology of storage and lytic vacuoles, and the trafficking toward the vacuoles was defective. Moreover, multiple sac knockout mutants had an increased number of smaller storage and lytic vacuoles, whereas extralarge vacuoles were observed in the overexpression lines, correlating with various growth and developmental defects. The fragmented vacuolar phenotype of sac mutants could be mimicked by treating wild-type seedlings with PtdIns(3,5)P2, corroborating that this PPI is important for vacuole morphology. Taken together, these results provide evidence that PPIs, together with their metabolic enzymes SAC2-SAC5, are crucial for vacuolar trafficking and for vacuolar morphology and function in plants.
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