ArticleProceedings of the National Academy of Sciences of the United States of America2014
Trimethylamine N-oxide metabolism by abundant marine heterotrophic bacteria.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers, 1 of them a synthesis that pooled it.
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Who cites it
69 citing papers in PubMed, 1 synthesis or guideline pooled it, 160 citations in OpenAlex.
- Modulation of Circulating Trimethylamine N-Oxide Concentrations by Dietary Supplements and Pharmacological Agents: A Systematic Review.Advances in nutrition (Bethesda, Md.) · 2019Pooled it
- Article
- Coordinated regulation of trimethylamine catabolism in abundant marine bacteria.Applied and environmental microbiology · 2026Article
- Factors Influencing Undesirable Sensory Properties in Protein Hydrolysates and Remediation Strategies for Food Applications.Journal of food science · 2026Review
- Monooxygenase-dehydrogenase cascade for sustained enzymatic remediation of TMA in salmon protein hydrolysates.Applied and environmental microbiology · 2026Article
- Calorimetric characterization of the stability and activity of trimethylamine-N-oxide (TMAO) demethylase from Methylocella silvestris BL2.Protein science : a publication of the Protein Society · 2025Article
- Methionine synthesis and glycine betaine demethylation are intricately intertwined in cosmopolitan marine bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Marine Community Metabolomes in the Eastern Tropical North Pacific Oxygen Deficient Zone Reveal Glycine Betaine as a Metabolic Link Between Prochlorococcus and SAR11.Environmental microbiology · 2025Article
- Screening and Isolation of Bacterial Strains Able to Degrade Trimethylamine.Microorganisms · 2025Article
- Trimethylamine-N-Oxide (TMAO) as a Rising-Star Metabolite: Implications for Human Health.Metabolites · 2025Review
- Microbial Community Metabolism of Coral Reef Exometabolomes Broadens the Chemodiversity of Labile Dissolved Organic Matter.Environmental microbiology · 2025Article
- Article
- Collaborative metabolic curation of an emerging model marine bacterium, Alteromonas macleodii ATCC 27126.PloS one · 2025Article
- Edible Coatings for Fish Preservation: Literature Data on Storage Temperature, Product Requirements, Antioxidant Activity, and Coating Performance-A Review.Antioxidants (Basel, Switzerland) · 2024Review
- Cardiovascular risk of dietary trimethylamine oxide precursors and the therapeutic potential of resveratrol and its derivatives.FEBS open bio · 2024Review
- Dietary betaine and/or TMAO affect hepatic lipid accumulation and glycometabolism of Megalobrama amblycephala exposed to a high-carbohydrate diet.Fish physiology and biochemistry · 2024Article
- Metabolic mediators: microbial-derived metabolites as key regulators of anti-tumor immunity, immunotherapy, and chemotherapy.Frontiers in immunology · 2024Review
- Bacterial catabolism of membrane phospholipids links marine biogeochemical cycles.Science advances · 2023Article
- Functional annotation and importance of marine bacterial transporters of plankton exometabolites.ISME communications · 2023Article
- Ubiquitous occurrence of a dimethylsulfoniopropionate ABC transporter in abundant marine bacteria.The ISME journal · 2023Article
9 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Trimethylamine N-oxide (TMAO) is a common osmolyte found in a variety of marine biota and has been detected at nanomolar concentrations in oceanic surface waters. TMAO can serve as an important nutrient for ecologically important marine heterotrophic bacteria, particularly the SAR11 clade and marine Roseobacter clade (MRC). However, the enzymes responsible for TMAO catabolism and the membrane transporter required for TMAO uptake into microbial cells have yet to be identified. We show here that the enzyme TMAO demethylase (Tdm) catalyzes the first step in TMAO degradation. This enzyme represents a large group of proteins with an uncharacterized domain (DUF1989). The function of TMAO demethylase in a representative from the SAR11 clade (strain HIMB59) and in a representative of the MRC (Ruegeria pomeroyi DSS-3) was confirmed by heterologous expression of tdm (the gene encoding Tdm) in Escherichia coli. In R. pomeroyi, mutagenesis experiments confirmed that tdm is essential for growth on TMAO. We also identified a unique ATP-binding cassette transporter (TmoXWV) found in a variety of marine bacteria and experimentally confirmed its specificity for TMAO through marker exchange mutagenesis and lacZ reporter assays of the promoter for genes encoding this transporter. Both Tdm and TmoXWV are particularly abundant in natural seawater assemblages and actively expressed, as indicated by a number of recent metatranscriptomic and metaproteomic studies. These data suggest that TMAO represents a significant, yet overlooked, nutrient for marine bacteria.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.