ArticleeLife2014
Single molecule FRET reveals pore size and opening mechanism of a mechano-sensitive ion channel.
Article in eLife, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
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Who cites it
81 citing papers in PubMed.
- Amino acid availability influences Staphylococcus aureus interaction with the nasal microbiota in a strain- and community-specific manner.Microbiome · 2026Article
- BRET-Based Approaches for Ion Channels: Emerging Applications and Future Directions.Archiv der Pharmazie · 2026Review
- Structural basis of the gating mechanism of the large-conductance mechanosensitive channel from Escherichia coli.Nature communications · 2026Article
- Bayesian-Steered Structure Prediction of Mechanical Biomolecules Using Twisted Diffusion.bioRxiv : the preprint server for biology · 2026Article
- Opening threshold and kinetics of the MscL mechanosensitive channel are regulated by its periplasmic loop.Research square · 2026Article
- Opening threshold and kinetics of the MscL mechanosensitive channel are regulated by its periplasmic loop.npj biological physics and mechanics · 2026Article
- Mechanism of capsaicin entry into buried vanilloid sites in TRPV1.Nature chemical biology · 2025Article
- Mechanomedicine: Present state and future promise.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Lipid interactions and gating hysteresis suggest a physiological role for mechanosensitive channel YnaI.Nature communications · 2025Article
- Asymmetric Nature of MscL Opening Revealed by Molecular Dynamics Simulations.Journal of chemical information and modeling · 2025Article
- Competitive fitness of Staphylococcus aureus against nasal commensals depends on biotin biosynthesis and acquisition.The ISME journal · 2025Article
- Force versus Response: Methods for Activating and Characterizing Mechanosensitive Ion Channels and GPCRs.Advanced healthcare materials · 2024Review
- Multifunctional Nanomaterials Mediate Cholesterol Depletion for Cancer Treatment.Angewandte Chemie (International ed. in English) · 2024Review
- Observing mechanosensitive channels in action in living bacteria.Biophysical reports · 2024Article
- Microbial membrane transport proteins and their biotechnological applications.World journal of microbiology & biotechnology · 2024Review
- Mechanoregulatory Cholesterol Oxidase-Functionalized Nanoscale Metal-Organic Framework Stimulates Pyroptosis and Reinvigorates T Cells.Small (Weinheim an der Bergstrasse, Germany) · 2023Article
- The Effects of Airflow on the Mechanosensitive Channels ofMicroorganisms · 2023Article
- Article
- Mechanosensitive aquaporins.Biophysical reviews · 2023Review
- Microscopic mechanism of PIEZO1 activation by pressure-induced membrane stretch.The Journal of general physiology · 2023Article
21 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
The mechanosensitive channel of large conductance, which serves as a model system for mechanosensitive channels, has previously been crystallized in the closed form, but not in the open form. Ensemble measurements and electrophysiological sieving experiments show that the open-diameter of the channel pore is >25 Å, but the exact size and whether the conformational change follows a helix-tilt or barrel-stave model are unclear. Here we report measurements of the distance changes on liposome-reconstituted MscL transmembrane α-helices, using a 'virtual sorting' single-molecule fluorescence energy transfer. We observed directly that the channel opens via the helix-tilt model and the open pore reaches 2.8 nm in diameter. In addition, based on the measurements, we developed a molecular dynamics model of the channel structure in the open state which confirms our direct observations. DOI: http://dx.doi.org/10.7554/eLife.01834.001.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.