ArticleeLife2014
An unmet actin requirement explains the mitotic inhibition of clathrin-mediated endocytosis.
Article in eLife, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 56 citations in OpenAlex.
- The Plasma Membrane and Mechanoregulation in Cells.ACS omega · 2024Review
- Research progress on the regulatory role of cell membrane surface tension in cell behavior.Heliyon · 2024Review
- Tension-induced adhesion mode switching: the interplay between focal adhesions and clathrin-containing adhesion complexes.bioRxiv : the preprint server for biology · 2024Article
- WAVE facilitates polarized E-cadherin transport.Molecular biology of the cell · 2023Article
- Article
- Branched actin networks are organized for asymmetric force production during clathrin-mediated endocytosis in mammalian cells.Nature communications · 2022Article
- Endocytosis at extremes: Formation and internalization of giant clathrin-coated pits under elevated membrane tension.Frontiers in molecular biosciences · 2022Article
- Tetraspanins: Host Factors in Viral Infections.International journal of molecular sciences · 2021Review
- Dynamic interplay between cell membrane tension and clathrin-mediated endocytosis.Biology of the cell · 2021Review
- Review
- Regulation of the Total Cell Surface Area in DividingFrontiers in cell and developmental biology · 2020Article
- Clathrin heavy chain phosphorylated at T606 plays a role in proper cell division.Cell cycle (Georgetown, Tex.) · 2019Article
- Local actin nucleation tunes centrosomal microtubule nucleation during passage through mitosis.The EMBO journal · 2019Article
- Article
- Mechanisms of clathrin-mediated endocytosis.Nature reviews. Molecular cell biology · 2018Review
- Membrane bending by actin polymerization.Current opinion in cell biology · 2018Review
- New tools for "hot-wiring" clathrin-mediated endocytosis with temporal and spatial precision.The Journal of cell biology · 2017Article
- Mechanoregulation of clathrin-mediated endocytosis.Journal of cell science · 2017Article
- Membrane dynamics of dividing cells imaged by lattice light-sheet microscopy.Molecular biology of the cell · 2016Article
- Deciphering dynamics of clathrin-mediated endocytosis in a living organism.The Journal of cell biology · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Clathrin-mediated endocytosis (CME) is the major internalisation route for many different receptor types in mammalian cells. CME is shut down during early mitosis, but the mechanism of this inhibition is unclear. In this study, we show that the mitotic shutdown is due to an unmet requirement for actin in CME. In mitotic cells, membrane tension is increased and this invokes a requirement for the actin cytoskeleton to assist the CME machinery to overcome the increased load. However, the actin cytoskeleton is engaged in the formation of a rigid cortex in mitotic cells and is therefore unavailable for deployment. We demonstrate that CME can be 'restarted' in mitotic cells despite high membrane tension, by allowing actin to engage in endocytosis. Mitotic phosphorylation of endocytic proteins is maintained in mitotic cells with restored CME, indicating that direct phosphorylation of the CME machinery does not account for shutdown. DOI: http://dx.doi.org/10.7554/eLife.00829.001.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.