ArticleDevelopment (Cambridge, England)2014
A targeted gene expression system using the tryptophan repressor in zebrafish shows no silencing in subsequent generations.
Article in Development (Cambridge, England), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Versatile CRISPR-Cas Tools for Gene Regulation in Zebrafish via an Enhanced Q Binary System.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Whole-brain imaging of freely-moving zebrafish.Frontiers in neuroscience · 2023Review
- Non-telecentric two-photon microscopy for 3D random access mesoscale imaging.Nature communications · 2022Article
- The Q-system: A Versatile Repressible Binary Expression System.Methods in molecular biology (Clifton, N.J.) · 2022Review
- IQ-Switch is a QF-based innocuous, silencing-free, and inducible gene switch system in zebrafish.Communications biology · 2021Article
- Ancestral circuits for vertebrate color vision emerge at the first retinal synapse.Science advances · 2021Article
- Zebrafish Retinal Ganglion Cells Asymmetrically Encode Spectral and Temporal Information across Visual Space.Current biology : CB · 2020Article
- Fovea-like Photoreceptor Specializations Underlie Single UV Cone Driven Prey-Capture Behavior in Zebrafish.Neuron · 2020Article
- Osteoarthritis-Like Changes in Bardet-Biedl Syndrome Mutant Ciliopathy Mice (Frontiers in physiology · 2018Article
- Hearing sensitivity differs between zebrafish lines used in auditory research.Hearing research · 2016Article
- Innervation regulates synaptic ribbons in lateral line mechanosensory hair cells.Journal of cell science · 2016Article
- Remote z-scanning with a macroscopic voice coil motor for fast 3D multiphoton laser scanning microscopy.Biomedical optics express · 2016Article
- Presynaptic partner selection during retinal circuit reassembly varies with timing of neuronal regeneration in vivo.Nature communications · 2016Article
- Transcriptional regulation using the Q system in transgenic zebrafish.Methods in cell biology · 2016Article
- Natural Bizbenzoquinoline Derivatives Protect Zebrafish Lateral Line Sensory Hair Cells from Aminoglycoside Toxicity.Frontiers in cellular neuroscience · 2016Article
- Defects of the Glycinergic Synapse in Zebrafish.Frontiers in molecular neuroscience · 2016Review
- CRISPR/Cas9-mediated conversion of eGFP- into Gal4-transgenic lines in zebrafish.Nature protocols · 2014Article
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4 authors.
Funding
Abstract
The ability to visualize and manipulate cell fate and gene expression in specific cell populations has made gene expression systems valuable tools in developmental biology studies. Here, we describe a new system that uses the E. coli tryptophan repressor and its upstream activation sequence (TrpR/tUAS) to drive gene expression in stable zebrafish transgenic lines and in mammalian cells. We show that TrpR/tUAS transgenes are not silenced in subsequent generations of zebrafish, which is a major improvement over some of the existing systems, such as Gal4/gUAS and the Q-system. TrpR transcriptional activity can be tuned by mutations in its DNA-binding domain, or silenced by Gal80 when fused to the Gal4 activation domain. In cases in which more than one cell population needs to be manipulated, TrpR/tUAS can be used in combination with other, existing systems.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.