ArticleMolecular and cellular biology2014
Screening with a novel cell-based assay for TAZ activators identifies a compound that enhances myogenesis in C2C12 cells and facilitates muscle repair in a muscle injury model.
Article in Molecular and cellular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 57 citations in OpenAlex.
- BET inhibition curbs macrophage inflammation, lipid accumulation, and atherogenesis by disrupting the YAP/TAZ-BRD4 axis.Journal of leukocyte biology · 2026Article
- Caffeic acid phenethyl ester ameliorates high-fat diet-induced muscle textural deterioration in grass carp (Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2026Article
- Signaling networks governing skeletal muscle growth, atrophy, and cachexiaSkeletal muscle · 2025Review
- Crosstalk among canonical Wnt and Hippo pathway members in skeletal muscle and at the neuromuscular junction.Neural regeneration research · 2025Article
- IBS008738, a TAZ activator, facilitates muscle repair and inhibits muscle injury in a mouse model of sport-induced injury.Cytotechnology · 2025Article
- Contractile regulation of the glucocorticoid-sensitive transcriptome in young and aged skeletal muscle.American journal of physiology. Endocrinology and metabolism · 2024Article
- A highly sensitive reporter system to monitor endogenous YAP1/TAZ activity and its application in various human cells.Cancer science · 2024Article
- Semaphorins: Missing Signals in Age-dependent Alteration of Neuromuscular Junctions and Skeletal Muscle Regeneration.Aging and disease · 2024Review
- In Vitro and In Vivo Evidence towards Fibronectin's Protective Effects against Prion Infection.International journal of molecular sciences · 2023Article
- Protein kinase Cα activation switches YAP1 from TEAD-mediated signaling to p73-mediated signaling.Cancer science · 2022Article
- MESH1 knockdown triggers proliferation arrest through TAZ repression.Cell death & disease · 2022Article
- The Neuromuscular Junction: Roles in Aging and Neuromuscular Disease.International journal of molecular sciences · 2021Review
- Yap regulates skeletal muscle fatty acid oxidation and adiposity in metabolic disease.Nature communications · 2021Article
- Effects of mechanical trauma on the differentiation and ArfGAP3 expression of C2C12 myoblast and mouse levator ani muscle.International urogynecology journal · 2020Article
- The RAS-interacting chaperone UNC119 drives the RASSF6-MDM2-p53 axis and antagonizes RAS-mediated malignant transformation.The Journal of biological chemistry · 2020Article
- RIPK3 upregulation confers robust proliferation and collateral cystine-dependence on breast cancer recurrence.Cell death and differentiation · 2020Article
- Article
- TAZ and myostatin involved in muscle atrophy of congenital neurogenic clubfoot.World journal of clinical cases · 2019Article
- TAZ activation by Hippo pathway dysregulation induces cytokine gene expression and promotes mesothelial cell transformation.Oncogene · 2019Article
- Doublecortin-like kinase 1 compromises DNA repair and induces chromosomal instability.Biochemistry and biophysics reports · 2018Article
Corrections and comments
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Authors and funding
14 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The transcriptional coactivator with a PDZ-binding motif (TAZ) cooperates with various transcriptional factors and plays various roles. Immortalized human mammalian epithelial MCF10A cells form spheres when TAZ is overexpressed and activated. We developed a cell-based assay using sphere formation by TAZ-expressing MCF10A cells as a readout to screen 18,458 chemical compounds for TAZ activators. Fifty compounds were obtained, and 47 were confirmed to activate the TAZ-dependent TEAD-responsive reporter activity in HEK293 cells. We used the derived subset of compounds as a TAZ activator candidate minilibrary and searched for compounds that promote myogenesis in mouse C2C12 myoblast cells. In this study, we focused on one compound, IBS008738. IBS008738 stabilizes TAZ, increases the unphosphorylated TAZ level, enhances the association of MyoD with the myogenin promoter, upregulates MyoD-dependent gene transcription, and competes with myostatin in C2C12 cells. TAZ knockdown verifies that the effect of IBS008738 depends on endogenous TAZ in C2C12 cells. IBS008738 facilitates muscle repair in cardiotoxin-induced muscle injury and prevents dexamethasone-induced muscle atrophy. Thus, this cell-based assay is useful to identify TAZ activators with a variety of cellular outputs. Our findings also support the idea that TAZ is a potential therapeutic target for muscle atrophy.
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