ArticlePhotosynthesis research2014
Isolation and characterization of novel high-CO2-requiring mutants of Chlamydomonas reinhardtii.
Article in Photosynthesis research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 28 citations in OpenAlex.
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- Prospects for Engineering Biophysical COAnnual review of plant biology · 2020Review
- Pyrenoid Starch Sheath Is Required for LCIB Localization and the COPlant physiology · 2020Article
- Elucidation and genetic intervention of COJournal of biosciences · 2020Article
- High-resolution suborganellar localization of CaProtoplasma · 2018Article
- Ion and metabolite transport in the chloroplast of algae: lessons from land plants.Cellular and molecular life sciences : CMLS · 2018Review
- The carbonic anhydrase CAH1 is an essential component of the carbon-concentrating mechanism inProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Protein Delivery into Plant Cells: TowardFrontiers in plant science · 2017Article
- Chloroplast-mediated regulation of CO2-concentrating mechanism by Ca2+-binding protein CAS in the green alga Chlamydomonas reinhardtii.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Characterization of cooperative bicarbonate uptake into chloroplast stroma in the green alga Chlamydomonas reinhardtii.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Algal dual-specificity tyrosine phosphorylation-regulated kinase, triacylglycerol accumulation regulator1, regulates accumulation of triacylglycerol in nitrogen or sulfur deficiency.Plant physiology · 2015Article
- Article
- CCM8: the eighth international symposium on inorganic carbon uptake by aquatic photosynthetic organisms.Photosynthesis research · 2014Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aquatic microalgae induce a carbon-concentrating mechanism (CCM) to maintain photosynthetic activity in low-CO2 (LC) conditions. Although the molecular mechanism of the CCM has been investigated using the single-cell green alga Chlamydomonas reinhardtii, and several CCM-related genes have been identified by analyzing high-CO2 (HC)-requiring mutants, many aspects of the CO2-signal transduction pathways remain to be elucidated. In this study, we report the isolation of novel HC-requiring mutants defective in the induction of CCM by DNA tagging. Growth rates of 20,000 transformants grown under HC and LC conditions were compared, and three HC-requiring mutants (H24, H82, and P103) were isolated. The photosynthetic CO2-exchange activities of these mutants were significantly decreased compared with that of wild-type cells, and accumulation of HLA3 and both LCIA and HLA3 were absent in mutants H24 and H82, respectively. Although the insertion of the marker gene and the HC-requiring phenotype were linked in the tetrad progeny of H82, and a calcium-sensing receptor CAS was disrupted by the insertion, exogenous expression of CAS alone could not complement the HC-requiring phenotype.
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