ArticleMethods in molecular biology (Clifton, N.J.)2014
In vitro systems for coupling RNAP II transcription to splicing and polyadenylation.
Article in Methods in molecular biology (Clifton, N.J.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed.
- A slow transcription rate causes embryonic lethality and perturbs kinetic coupling of neuronal genes.The EMBO journal · 2019Article
- Identification of new branch points and unconventional introns in Saccharomyces cerevisiae.RNA (New York, N.Y.) · 2016Article
- FUS functions in coupling transcription to splicing by mediating an interaction between RNAP II and U1 snRNP.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Primary microRNA processing is functionally coupled to RNAP II transcription in vitro.Scientific reports · 2015Article
- Utilizing functional cell-free extracts to dissect ribonucleoprotein complex biology at single-molecule resolution.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Studies over the past several years have revealed that steps in gene expression are extensively coupled to one another both physically and functionally. Recently, in vitro systems were developed for understanding the mechanisms involved in coupling transcription by RNA polymerase II to RNA processing. Here we describe an efficient two-way system for coupling transcription to splicing and a robust three-way system for coupling transcription, splicing, and polyadenylation. In these systems a CMV-DNA construct is incubated in HeLa cell nuclear extracts in the presence of (32)P-UTP to generate the nascent transcript. Transcription is then stopped by addition of α-amanitin followed by continued incubation to allow RNA processing.
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