ArticleMethods in molecular biology (Clifton, N.J.)2014
In vitro assay of pre-mRNA splicing in mammalian nuclear extract.
Article in Methods in molecular biology (Clifton, N.J.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Article
- Aberrant RNA methylation triggers recruitment of an alkylation repair complex.Molecular cell · 2021Article
- Article
- Development of an in vitro pre-mRNA splicing assay using plant nuclear extract.Plant methods · 2018Article
- Preparation of Splicing Competent Nuclear Extract from Mammalian Cells and In Vitro Pre-mRNA Splicing Assay.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Release of SR Proteins from CLK1 by SRPK1: A Symbiotic Kinase System for Phosphorylation Control of Pre-mRNA Splicing.Molecular cell · 2016Article
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Authors and funding
3 authors.
Funding
Abstract
The in vitro splicing assay is a valuable technique that can be used to study the mechanism and machinery involved in the splicing process. The ability to investigate various aspects of splicing and alternative splicing appears to be endless due to the flexibility of this assay. Here, we describe the tools and techniques necessary to carry out an in vitro splicing assay. Through the use of radiolabeled pre-mRNA and crude nuclear extract, spliced mRNAs can be purified and visualized by autoradiography for downstream analysis.
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