ArticleMolecular and cellular biology1988
Cloning of the human keratin 18 gene and its expression in nonepithelial mouse cells.
Article in Molecular and cellular biology, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 111 citations in OpenAlex.
- The relationship between keratin 18 and epithelial-derived tumors: as a diagnostic marker, prognostic marker, and its role in tumorigenesis.Frontiers in oncology · 2024Review
- Hypomethylating Agent Azacitidine Is Effective in Treating Brain Metastasis Triple-Negative Breast Cancer Through Regulation of DNA Methylation of Keratin 18 Gene.Translational oncology · 2020Article
- Allele-specific recognition by LILRB3 and LILRA6 of a cytokeratin 8-associated ligand on necrotic glandular epithelial cells.Oncotarget · 2016Article
- A cell culture system for the induction of Mallory bodies: Mallory bodies and aggresomes represent different types of inclusion bodies.Histochemistry and cell biology · 2009Article
- Differential sensitivity of mouse epithelial tissues to the polyomavirus middle T oncogene.The American journal of pathology · 2006Article
- Identification of novel principles of keratin filament network turnover in living cells.Molecular biology of the cell · 2004Article
- Intermediate cells in human prostate epithelium are enriched in proliferative inflammatory atrophy.The American journal of pathology · 2003Article
- Expression of conditional cre recombinase in epithelial tissues of transgenic mice.Genesis (New York, N.Y. : 2000) · 2003Article
- Complete cytolysis and neonatal lethality in keratin 5 knockout mice reveal its fundamental role in skin integrity and in epidermolysis bullosa simplex.Molecular biology of the cell · 2001Article
- Stromelysin-1 regulates adipogenesis during mammary gland involution.The Journal of cell biology · 2001Article
- The functional diversity of epidermal keratins revealed by the partial rescue of the keratin 14 null phenotype by keratin 16.The Journal of cell biology · 1999Article
- LCC15-MB: a vimentin-positive human breast cancer cell line from a femoral bone metastasis.Clinical & experimental metastasis · 1999Article
- Transcription factor Sp1 activates involucrin promoter activity in non-epithelial cell types.The Biochemical journal · 1999Article
- Regulation of keratin and integrin gene expression in cancer and drug resistance.Cytotechnology · 1998Article
- Caspase cleavage of keratin 18 and reorganization of intermediate filaments during epithelial cell apoptosis.The Journal of cell biology · 1997Article
- Rescue of mammary epithelial cell apoptosis and entactin degradation by a tissue inhibitor of metalloproteinases-1 transgene.The Journal of cell biology · 1996Article
- Oncogenic regulation and function of keratins 8 and 18.Cancer metastasis reviews · 1996Review
- The basal keratin network of stratified squamous epithelia: defining K15 function in the absence of K14.The Journal of cell biology · 1995Article
- An Sp1 binding site and the minimal promoter contribute to overexpression of the cytokeratin 18 gene in tumorigenic clones relative to that in nontumorigenic clones of a human carcinoma cell line.Molecular and cellular biology · 1995Article
- Expression of an epidermal keratin protein in liver of transgenic mice causes structural and functional abnormalities.The Journal of cell biology · 1995Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Human keratin 18 (K18) and the homologous mouse protein, Endo B, are intermediate filament subunits of the type I keratin class. Both are expressed in many simple epithelial cell types including trophoblasts, the first differentiated cell type to appear during mouse embryogenesis. The K18 gene was identified and cloned from among the 15 to 20 similar sequences identified within the human genome. The identity of the cloned gene was confirmed by comparing the sequence of the first two exons to the K18 cDNA sequence and transfecting the gene into various murine cell lines and verifying the encoded protein as K18 by immunoprecipitation and partial peptide mapping. The transfected K18 gene was expressed in mouse HR9 parietal endodermal cells and mouse fibroblasts even though the fibroblasts fail to express endogenous Endo B. S1 nuclease protection analysis indicated that mRNA synthesized from the transfected K18 gene is initiated at the same position as authentic K18 mRNA found in both BeWo trophoblastoma cells and HeLa cells. Pulse-chase experiments indicated that the human K18 protein is stable in murine parietal endodermal cells (HR9) which express EndoA, a complementary mouse type II keratin. Surprisingly, however, K18 was degraded when synthesized in cells which lack a type II keratin. This turnover of K18 may be an important mechanism by which epithelial cells maintain equal molar amounts of both type I and II keratins. In addition, the levels of the endogenous type I Endo B in parietal endodermal cells were compensatingly down regulated in the presence of the K18 protein, while the levels of the endogenous type II Endo A were not affected in any of the transfected cell lines.
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