ArticleBMC microbiology2014
Identification of type III secretion substrates of Chlamydia trachomatis using Yersinia enterocolitica as a heterologous system.
Article in BMC microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed.
- A new core-genome MLST approach for Chlamydia trachomatis genomic surveillance and research.Nature communications · 2026Article
- ThemBio · 2026Article
- A lineage-specific heat-induced feedback loop controls HrcA to promote chlamydial fitness under stress.bioRxiv : the preprint server for biology · 2025Article
- A conservedMicrobiology (Reading, England) · 2025Article
- Lawsonia intracellularis T3SS effector LI0758, an Rce1 ortholog, activates MAPK and NF-κB signaling in mammalian cells.Veterinary research · 2025Article
- Isolation and characterization of a Chlamydia muridarum tc0237 mutant from a genetic screen that is attenuated in epithelial cells.PloS one · 2025Article
- Pangenome-Wide Association Study in theInternational journal of molecular sciences · 2024Article
- Identification of homologs of the Chlamydia trachomatis effector CteG reveals a family of Chlamydiaceae type III secreted proteins that can be delivered into host cells.Medical microbiology and immunology · 2024Article
- Characterization of translocon proteins in the type III secretion system of Lawsonia intracellularis.Veterinary research · 2023Article
- Type III Secretion inMicrobiology and molecular biology reviews : MMBR · 2023Review
- Identification and Preliminary Characterization of Novel Type III Secreted Effector Proteins in Chlamydia trachomatis.Infection and immunity · 2023Article
- Virulence Protein Pgp3 Is Insufficient To Mediate Plasmid-Dependent Infectivity of Chlamydia trachomatis.Infection and immunity · 2023Article
- In Search of a Mechanistic Link between Chlamydia trachomatis-Induced Cellular Pathophysiology and Oncogenesis.Infection and immunity · 2023Review
- Lawsonia intracellularis LI0666 is a new EPIYA effector exported by the Yersinia enterocolitica type III secretion system.Veterinary research · 2022Article
- The Type III Secretion Effector CteG Mediates Host Cell Lytic Exit ofFrontiers in cellular and infection microbiology · 2022Article
- Application of aJournal of bacteriology · 2021Article
- A Same-Genus Screening Approach Reveals Novel Effectors and New Possibilities for InvestigatingJournal of bacteriology · 2021Article
- Unrelated Fungal Rust Candidate Effectors Act on Overlapping Plant Functions.Microorganisms · 2021Article
- Got mutants? How advances in chlamydial genetics have furthered the study of effector proteins.Pathogens and disease · 2021Review
- Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundChlamydia trachomatis is an obligate intracellular human pathogen causing ocular and urogenital infections that are a significant clinical and public health concern. This bacterium uses a type III secretion (T3S) system to manipulate host cells, through the delivery of effector proteins into their cytosol, membranes, and nucleus. In this work, we aimed to find previously unidentified C. trachomatis T3S substrates.
resultsWe first analyzed the genome of C. trachomatis L2/434 strain for genes encoding mostly uncharacterized proteins that did not appear to possess a signal of the general secretory pathway and which had not been previously experimentally shown to be T3S substrates. We selected several genes with these characteristics and analyzed T3S of the encoding proteins using Yersinia enterocolitica as a heterologous system. We identified 23 C. trachomatis proteins whose first 20 amino acids were sufficient to drive T3S of the mature form of β-lactamase TEM-1 by Y. enterocolitica. We found that 10 of these 23 proteins were also type III secreted in their full-length versions by Y. enterocolitica, providing additional support that they are T3S substrates. Seven of these 10 likely T3S substrates of C. trachomatis were delivered by Y. enterocolitica into host cells, further suggesting that they could be effectors. Finally, real-time quantitative PCR analysis of expression of genes encoding the 10 likely T3S substrates of C. trachomatis showed that 9 of them were clearly expressed during infection of host cells.
conclusionsUsing Y. enterocolitica as a heterologous system, we identified 10 likely T3S substrates of C. trachomatis (CT053, CT105, CT142, CT143, CT144, CT161, CT338, CT429, CT656, and CT849) and could detect translocation into host cells of CT053, CT105, CT142, CT143, CT161, CT338, and CT429. Therefore, we revealed several C. trachomatis proteins that could be effectors subverting host cell processes.
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