ArticleBMC veterinary research2014
Development of a direct competitive ELISA for the detection of Mycoplasma bovis infection based on a monoclonal antibody of P48 protein.
Article in BMC veterinary research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- Isolation, Identification, and Molecular Characterization ofPathogens (Basel, Switzerland) · 2026Article
- Evaluation of a P48 ELISA for Mycoplasmopsis (Mycoplasma) bovis in North American bison (Bison bison): inferior performance compared to a commercially available ELISA.BMC veterinary research · 2026Article
- Technologies in Biomarker Discovery for Animal Diseases: Mechanisms, Classification, and Diagnostic Applications.Animals : an open access journal from MDPI · 2025Review
- Evaluation ofInfection and immunity · 2025Article
- Fusion membrane proteins derived subunit vaccine candidates effectively protect against Mycoplasma bovis challenge in mice.BMC veterinary research · 2025Article
- Review
- Point-of-care potentials of lateral flow-based field screening forBiology methods & protocols · 2024Review
- Molecular mechanism of Dang-Shen-Yu-Xing decoction againstFrontiers in veterinary science · 2024Article
- A novel phage-displayed MilA ELISA for detection of antibodies against Myc. bovis in bovine milk.Journal of applied microbiology · 2022Article
- MBOVPG45_0375 Encodes an IgG-Binding Protein and MBOVPG45_0376 Encodes an IgG-Cleaving Protein inFrontiers in veterinary science · 2021Article
- Development and application of a colloidal carbon test strip for the detection of antibodies against Mycoplasma bovis.World journal of microbiology & biotechnology · 2020Article
- A European inter-laboratory trial to evaluate the performance of three serological methods for diagnosis of Mycoplasma bovis infection in cattle using latent class analysis.BMC veterinary research · 2019Article
- A review of mycoplasma diagnostics in cattle.Journal of veterinary internal medicine · 2018Review
- The Performance of Three Immune Assays to Assess the Serological Status of Cattle Experimentally Exposed to Mycoplasma bovis.Veterinary sciences · 2018Article
- Mechanism of Apoptosis Induction by Mycoplasmal Nuclease MGA_0676 in Chicken Embryo Fibroblasts.Frontiers in cellular and infection microbiology · 2018Article
- Immunoproteomic identification of MbovP579, a promising diagnostic biomarker for serological detection of Mycoplasma bovis infection.Oncotarget · 2016Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMycoplasma bovis (M. bovis) is a major, but often overlooked, pathogen documented to cause respiratory disease, mastitis, and arthritis in cattle throughout China since 2008. Here, we report the development of a direct competitive enzyme-linked immunosorbent assay (Dc-ELISA) to detect M. bovis antibody.
resultsWe used a recombinant P48 protein and monoclonal antibody (mAb) 10E. MAb 10E, prepared against the recombinant P48 protein of M. bovis, identified all M. bovis strains with no cross-reactivity with other related pathogens. Coating micro plates with P48 protein instead of whole M. bovis cells as well as the use of mAb 10E produced a specific and sensitive Dc-ELISA for M. bovis antibody detection with a cut-off percent inhibition (PI) value of 32%. Compared with two commercial indirect ELISA (i-ELISA) kits, our Dc-ELISA offered a higher positive detection rate in 165 clinical bovine serum samples.
conclusionsA rapid, sensitive, and reliable serological diagnosis method was developed for M. bovis, which can facilitate M. bovis surveillance, assisting researchers in understanding the ecology and epidemiology of M. bovis.
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