ArticlePloS one2014
Time-dependent expression profiles of microRNAs and mRNAs in rat milk whey.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
41 citing papers in PubMed, 74 citations in OpenAlex.
- Human Breast Milk miRNA, Maternal Probiotic Supplementation and Atopic Dermatitis in Offspring.PloS one · 2015Trial
- Human Milk MiRNA Acts on Infections in Both the Maternal Body and the Child During Breastfeeding.Life (Basel, Switzerland) · 2026Article
- Characterization and biological health implications of microRNAs from human milk and infant formulas.NPJ science of food · 2025Review
- Human milk-derived extracellular vesicles promote the heat shock response in polarized microglia.Cell stress & chaperones · 2025Article
- Comparative miRNome analysis of colostrum- and mature milk-derived extracellular vesicles from Holstein and Jersey cows.Journal of animal science and technology · 2025Article
- Changes in and relationships between human milk oligosaccharides and microRNAs in milk-derived extracellular vesicles during the first 4 months of lactation.Frontiers in nutrition · 2025Article
- Milk derived extracellular vesicle uptake in human microglia regulates the DNA methylation machinery : Short title: milk-derived extracellular vesicles and the epigenetic machinery.Scientific reports · 2024Article
- Recent insights into breast milk microRNA: their role as functional regulators.Frontiers in nutrition · 2024Review
- Effects of Cow's Milk Processing on MicroRNA Levels.Foods (Basel, Switzerland) · 2023Article
- The contributions of parental lactation on offspring development: It's not udder nonsense!Hormones and behavior · 2023Review
- The Protective Role of Exosome-Derived MicroRNAs and Proteins from Human Breast Milk against Infectious Agents.Metabolites · 2023Article
- Human Milk-Derived Levels of let-7g-5p May Serve as a Diagnostic and Prognostic Marker of Low Milk Supply in Breastfeeding Women.Nutrients · 2023Article
- Extracellular vesicles and exosome-like nanovesicles as pioneering oral drug delivery systems.Frontiers in bioengineering and biotechnology · 2023Review
- Role of Hormones During Gestation and Early Development: Pathways Involved in Developmental Programming.Advances in experimental medicine and biology · 2023Article
- The Therapeutic Potential of Milk Extracellular Vesicles on Colorectal Cancer.International journal of molecular sciences · 2022Review
- Transcriptomic Characterization of Cow, Donkey and Goat Milk Extracellular Vesicles Reveals Their Anti-Inflammatory and Immunomodulatory Potential.International journal of molecular sciences · 2021Article
- Comparison of methods for pre-processing, exosome isolation, and RNA extraction in unpasteurized bovine and human milk.PloS one · 2021Article
- Milk Exosomes: Perspective Agents for Anticancer Drug Delivery.International journal of molecular sciences · 2020Review
- MicroRNA Milk Exosomes: From Cellular Regulator to Genomic Marker.Animals : an open access journal from MDPI · 2020Review
- Oral Administration of Bovine and Porcine Milk Exosome Alter miRNAs Profiles in Piglet Serum.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Functional RNAs, such as microRNA (miRNA) and mRNA, are present in milk, but their roles are unknown. To clarify the roles of milk RNAs, further studies using experimental animals such as rats are needed. However, it is unclear whether rat milk also contains functional RNAs and what their time dependent expression profiles are. Thus, we prepared total RNA from whey isolated from rat milk collected on days 2, 9, and 16 postpartum and analyzed using microarrays and quantitative PCR. The concentration of RNA in colostrum whey (day 2) was markedly higher than that in mature milk whey (days 9 and 16). Microarray analysis detected 161 miRNAs and 10,948 mRNA transcripts. Most of the miRNAs and mRNA transcripts were common to all tested milks. Finally, we selected some immune- and development-related miRNAs and mRNAs, and analysed them by quantitative PCR (in equal sample volumes) to determine their time-dependent changes in expression in detail. Some were significantly more highly expressed in colostrum whey than in mature milk whey, but some were expressed equally. And mRNA expression levels of some cytokines and hormones did not reflect the protein levels. It is still unknown whether RNAs in milk play biological roles in neonates. However, our data will help guide future in vivo studies using experimental animals such as rats.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.