ArticleThe Plant cell2014
Profiling of translatomes of in vivo-grown pollen tubes reveals genes with roles in micropylar guidance during pollination in Arabidopsis.
Article in The Plant cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 62 citations in OpenAlex.
- Translational and epitranscriptomic regulation of seed germination in Arabidopsis thaliana genotypes with contrasting dormancy phenotypes.Plant molecular biology · 2025Article
- The Crucial Roles of Phloem Companion Cells in Response to Phosphorus Deficiency.Plant, cell & environment · 2025Article
- Regulatory dynamics of gene expression in the developing male gametophyte of Arabidopsis.Plant reproduction · 2023Article
- Heat stress response mechanisms in pollen development.The New phytologist · 2021Review
- Persistent directional growth capability in Arabidopsis thaliana pollen tubes after nuclear elimination from the apex.Nature communications · 2021Article
- The molecular signatures of compatible and incompatible pollination in Arabidopsis.BMC genomics · 2021Article
- Translational gene regulation in plants: A green new deal.Wiley interdisciplinary reviews. RNA · 2020Review
- Fertilization in flowering plants: an odyssey of sperm cell delivery.Plant molecular biology · 2020Review
- Translational regulation contributes to the elevated COThe Plant journal : for cell and molecular biology · 2020Article
- Reactive Oxygen Species as Mediators of Gametophyte Development and Double Fertilization in Flowering Plants.Frontiers in plant science · 2020Review
- Extensive profiling inRNA (New York, N.Y.) · 2019Article
- Transcriptomics of Arabidopsis sperm cells at single-cell resolution.Plant reproduction · 2019Article
- Identification of phloem-associated translatome alterations during leaf development inHorticulture research · 2019Article
- Computational and Experimental Tools to Monitor the Changes in Translation Efficiency of Plant mRNA on a Genome-Wide Scale: Advantages, Limitations, and Solutions.International journal of molecular sciences · 2018Review
- SMALL AUXIN UP RNA62/75 Are Required for the Translation of Transcripts Essential for Pollen Tube Growth.Plant physiology · 2018Article
- Dynamics of the Pollen Sequestrome Defined by Subcellular Coupled Omics.Plant physiology · 2018Article
- Multilayered signaling pathways for pollen tube growth and guidance.Plant reproduction · 2018Review
- Cell-cell communications and molecular mechanisms in plant sexual reproduction.Journal of plant research · 2018Review
- Transcriptome dynamics revealed by a gene expression atlas of the early Arabidopsis embryo.Nature plants · 2017Article
- Mutations in eIF5B Confer Thermosensitive and Pleiotropic Phenotypes via Translation Defects inThe Plant cell · 2017Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcriptome profiling has been used to identify genes expressed in pollen tubes elongating in vitro; however, little is known of the transcriptome of in vivo-grown pollen tubes due to the difficulty of collecting pollen that is elongating within the solid maternal gynoecium. Using a pollen-specific promoter (ProLAT52) to generate epitope-tagged polysomal-RNA complexes that could be affinity purified, we obtained mRNAs undergoing translation (the translatome) of in vivo-grown pollen tubes from self-pollinated gynoecia of Arabidopsis thaliana. Translatomes of pollen grains as well as in vivo- and in vitro-cultured pollen tubes were assayed by microarray analyses, revealing over 500 transcripts specifically enriched in in vivo-elongating pollen tubes. Functional analyses of several in vivo mutants (iv) of these pollination-enhanced transcripts revealed partial pollination/fertilization and seed formation defects in siliques (iv2, iv4, and iv6). Cytological observation confirmed the involvement of these genes in specialized processes including micropylar guidance (IV6 and IV4), pollen tube burst (IV2), and repulsion of multiple pollen tubes in embryo sac (IV2). In summary, the selective immunopurification of transcripts engaged with polysomes in pollen tubes within self-fertilized florets has identified a cohort of pollination-enriched transcripts that facilitated the identification of genes important in in vivo pollen tube biology.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.