ArticleApplied and environmental microbiology2014
Role of malic enzyme during fatty acid synthesis in the oleaginous fungus Mortierella alpina.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 95 citations in OpenAlex.
- Linoleic Acid Production Platform via Mortierellaceae Strains: Bioprospection, Molecular Characterization, Cultivation Optimization, and Respirometric Analysis.Applied biochemistry and biotechnology · 2025Article
- Insights into the special physiology of Mortierella alpina cultured by agar supported solid state fermentation in enhancing arachidonic acid enriched lipid production.Scientific reports · 2025Article
- Early-Stage Infection-SpecificInternational journal of molecular sciences · 2024Article
- TA-Cloning for Diabetes Treatment: Expressing Corynebacterium Malic Enzyme Gene in E. coli.Current microbiology · 2024Article
- Potential of Mortierellaceae for polyunsaturated fatty acids production: mini review.Biotechnology letters · 2023Review
- Applications of Diacylglycerol Acyltransferase for Triacylglycerol Production inJournal of fungi (Basel, Switzerland) · 2023Article
- NADPH-Cytochrome P450 Reductase Mediates the Fatty Acid Desaturation of ω3 and ω6 Desaturases fromCurrent issues in molecular biology · 2022Article
- Role of Cytosolic Malic Enzyme in Oleaginicity of High-Lipid-Producing Fungal StrainJournal of fungi (Basel, Switzerland) · 2022Article
- Overexpression of the Mitochondrial Malic Enzyme Genes (Frontiers in microbiology · 2022Article
- Recent Molecular Tools for the Genetic Manipulation of Highly Industrially Important Mucoromycota Fungi.Journal of fungi (Basel, Switzerland) · 2021Review
- Advances in improving the biotechnological application of oleaginous fungus Mortierella alpina.Applied microbiology and biotechnology · 2021Review
- Role of the mitochondrial citrate-oxoglutarate carrier in lipid accumulation in the oleaginous fungus Mortierella alpina.Biotechnology letters · 2021Article
- Carbohydrate analysis of Mortierella alpina by colorimetry and HPLC-ELSD to reveal accumulation differences of sugar and lipid.Biotechnology letters · 2021Article
- Research advances on arachidonic acid production by fermentation and genetic modification of Mortierella alpina.World journal of microbiology & biotechnology · 2021Review
- Characterization of NADFrontiers in nutrition · 2021Article
- Engineering cofactor metabolism for improved protein and glucoamylase production in Aspergillus niger.Microbial cell factories · 2020Article
- Time-resolved multi-omics analysis reveals the role of nutrient stress-induced resource reallocation for TAG accumulation in oleaginous fungusBiotechnology for biofuels · 2020Article
- The Role of Glyceraldehyde-3-Phosphate Dehydrogenases in NADPH Supply in the Oleaginous Filamentous FungusFrontiers in microbiology · 2020Article
- Tetrahydrobiopterin Plays a Functionally Significant Role in Lipogenesis in the Oleaginous FungusFrontiers in microbiology · 2020Article
- Lipid and DHA-production in Aurantiochytrium sp. - Responses to nitrogen starvation and oxygen limitation revealed by analyses of production kinetics and global transcriptomes.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The generation of NADPH by malic enzyme (ME) was postulated to be a rate-limiting step during fatty acid synthesis in oleaginous fungi, based primarily on the results from research focusing on ME in Mucor circinelloides. This hypothesis is challenged by a recent study showing that leucine metabolism, rather than ME, is critical for fatty acid synthesis in M. circinelloides. To clarify this, the gene encoding ME isoform E from Mortierella alpina was homologously expressed. ME overexpression increased the fatty acid content by 30% compared to that for a control. Our results suggest that ME may not be the sole rate-limiting enzyme, but does play a role, during fatty acid synthesis in oleaginous fungi.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.