ArticleApplied and environmental microbiology2014
TiO2 photocatalysis damages lipids and proteins in Escherichia coli.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 209 citations in OpenAlex.
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- Biosynthesis of Peptide Hydrogel-Titania Nanoparticle Composites with Antibacterial Properties.Gels (Basel, Switzerland) · 2023Article
- Plasmon-Sensitized Silica-Titanium Aerogels as Potential Photocatalysts for Organic Pollutants and Bacterial Strains.ACS omega · 2023Article
- Antimicrobial Solutions for Endotracheal Tubes in Prevention of Ventilator-Associated Pneumonia.Materials (Basel, Switzerland) · 2023Review
- Study on the Possibilities of Developing Cementitious or Geopolymer Composite Materials with Specific Performances by Exploiting the Photocatalytic Properties of TiOMaterials (Basel, Switzerland) · 2023Review
- Novel Antibacterial Metals as Food Contact Materials: A Review.Materials (Basel, Switzerland) · 2023Review
- Photo-Fenton and TiOMolecules (Basel, Switzerland) · 2023Article
- Metal Oxide Nanoparticles: Review of Synthesis, Characterization and Biological Effects.Journal of functional biomaterials · 2022Review
- Evaluation of the antibacterial activities of face masks coated with titanium dioxide nanoparticles.Scientific reports · 2022Article
- Antifungal Effect of Nanoparticles against COVID-19 Linked Black Fungus: A Perspective on Biomedical Applications.International journal of molecular sciences · 2022Review
- Bioengineering Approaches to Fight against Orthopedic Biomaterials Related-Infections.International journal of molecular sciences · 2022Review
- Photoreactive Coating Material as an Effective and Durable Antimicrobial Composite in Reducing Bacterial Load on Surfaces in Livestock.Biomedicines · 2022Article
- Review
- Photocatalytic Treatments for Personal Protective Equipment: Experimental Microbiological Investigations and Perspectives for the Enhancement of Antimicrobial Activity by Micrometric TiOInternational journal of environmental research and public health · 2021Article
- Novel CuO/MnScientific reports · 2021Article
- Osmotic stress and vesiculation as key mechanisms controlling bacterial sensitivity and resistance to TiOCommunications biology · 2021Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study investigates the mechanisms of UV-A (315 to 400 nm) photocatalysis with titanium dioxide (TiO2) applied to the degradation of Escherichia coli and their effects on two key cellular components: lipids and proteins. The impact of TiO2 photocatalysis on E. coli survival was monitored by counting on agar plate and by assessing lipid peroxidation and performing proteomic analysis. We observed through malondialdehyde quantification that lipid peroxidation occurred during the photocatalytic process, and the addition of superoxide dismutase, which acts as a scavenger of the superoxide anion radical (O2·(-)), inhibited this effect by half, showing us that O2·(-) radicals participate in the photocatalytic antimicrobial effect. Qualitative analysis using two-dimensional electrophoresis allowed selection of proteins for which spot modifications were observed during the applied treatments. Two-dimensional electrophoresis highlighted that among the selected protein spots, 7 and 19 spots had already disappeared in the dark in the presence of 0.1 g/liter and 0.4 g/liter TiO2, respectively, which is accounted for by the cytotoxic effect of TiO2. Exposure to 30 min of UV-A radiation in the presence of 0.1 g/liter and 0.4 g/liter TiO2 increased the numbers of missing spots to 14 and 22, respectively. The proteins affected by photocatalytic oxidation were strongly heterogeneous in terms of location and functional category. We identified several porins, proteins implicated in stress response, in transport, and in bacterial metabolism. This study reveals the simultaneous effects of O2·(-) on lipid peroxidation and on the proteome during photocatalytic treatment and therefore contributes to a better understanding of molecular mechanisms in antibacterial photocatalytic treatment.
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Registered trials
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