ArticleApplied and environmental microbiology2014
A new subfamily of polyphosphate kinase 2 (class III PPK2) catalyzes both nucleoside monophosphate phosphorylation and nucleoside diphosphate phosphorylation.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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51 citing papers in PubMed, 123 citations in OpenAlex.
- Article
- The roles and synthesis of inorganic polyphosphate inbioRxiv : the preprint server for biology · 2026Article
- A Modular Nucleoside Kinase Cascade for the Synthesis of Ribonucleoside Triphosphates.Biotechnology and bioengineering · 2026Article
- ATP regeneration-driven biocatalytic production of 1,12-dodecanediol with improved cofactor supply.Bioresources and bioprocessing · 2026Article
- Polyphosphate acts as an architectural regulator of carbon fixation and nucleoid structure in cyanobacteria.bioRxiv : the preprint server for biology · 2026Article
- Identification of a distinct family of polyphosphate kinases relevant to energy homeostasis in Crenarchaeota.The Journal of biological chemistry · 2026Article
- Engineering of Corynebacterium glutamicum for the enhanced production of optically pure (meso)-2,3-butanediol.Bioresources and bioprocessing · 2026Article
- A universal polyphosphate kinase powers in vitro transcription.Nature communications · 2026Article
- Synthesis of 2'-fucosyllactose using multi-enzyme cascade with cofactor regeneration.Scientific reports · 2025Article
- Active Inclusion Bodies in the Multienzymatic Synthesis of UDP-N-acetylglucosamine.International journal of molecular sciences · 2025Article
- Polyphosphate from Lactic Acid Bacteria: A Functional Molecule for Food and Health Applications.Foods (Basel, Switzerland) · 2025Review
- Distribution of Polyphosphate Kinase 2 Genes in Bacteria Underscores a Dynamic Evolutionary History.Proteins · 2025Article
- Polyphosphate Kinase fromInternational journal of molecular sciences · 2024Article
- Fluoride and gallein regulate polyphosphate accumulation in dental caries-associatedMicrobiology (Reading, England) · 2024Article
- Chemo-enzymatic production of base-modified ATP analogues for polyadenylation of RNA.Chemical science · 2024Article
- An Update on Polyphosphate In Vivo Activities.Biomolecules · 2024Review
- Engineering the thermal stability of a polyphosphate kinase by ancestral sequence reconstruction to expand the temperature boundary for an industrially applicable ATP regeneration system.Applied and environmental microbiology · 2024Article
- Article
- Semirational engineering ofApplied and environmental microbiology · 2023Article
- Engineering sulfonate group donor regeneration systems to boost biosynthesis of sulfated compounds.Nature communications · 2023Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Inorganic polyphosphate (polyP) is a linear polymer of tens to hundreds of phosphate (Pi) residues linked by "high-energy" phosphoanhydride bonds as in ATP. PolyP kinases, responsible for the synthesis and utilization of polyP, are divided into two families (PPK1 and PPK2) due to differences in amino acid sequence and kinetic properties. PPK2 catalyzes preferentially polyP-driven nucleotide phosphorylation (utilization of polyP), which is important for the survival of microbial cells under conditions of stress or pathogenesis. Phylogenetic analysis suggested that the PPK2 family could be divided into three subfamilies (classes I, II, and III). Class I and II PPK2s catalyze nucleoside diphosphate and nucleoside monophosphate phosphorylation, respectively. Here, we demonstrated that class III PPK2 catalyzes both nucleoside monophosphate and nucleoside diphosphate phosphorylation, thereby enabling us to synthesize ATP from AMP by a single enzyme. Moreover, class III PPK2 showed broad substrate specificity over purine and pyrimidine bases. This is the first demonstration that class III PPK2 possesses both class I and II activities.
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