ArticleApplied and environmental microbiology2014
Characterization of temperate phages infecting Clostridium difficile isolates of human and animal origins.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 57 citations in OpenAlex.
- Structural determinants of SlpA-mediated phage recognition in Clostridioides difficile.PLoS pathogens · 2025Article
- Molecular mechanism of bacteriophage contraction structure of an S-layer-penetrating bacteriophage.Life science alliance · 2025Article
- Insight into the Mechanism of Lysogeny Control of phiCDKH01 Bacteriophage Infecting Clinical Isolate ofInternational journal of molecular sciences · 2024Article
- Novel insights into phage biology of the pathogenFrontiers in microbiology · 2024Article
- Emerging applications of phage therapy and fecal virome transplantation for treatment of Clostridioides difficile infection: challenges and perspectives.Gut pathogens · 2023Review
- Novel ribotype/sequence type associations and diverse CRISPR-Cas systems in environmental Clostridioides difficile strains from northern Iraq.FEMS microbiology letters · 2023Article
- The long and sinuous road to phage-based therapy ofFrontiers in medicine · 2023Review
- The Mechanism of Important Components in Canine Fecal Microbiota Transplantation.Veterinary sciences · 2022Review
- Whole-genome sequence analysis of Clostridium chauvoei isolated from clinical case of black quarter (BQ) from India.Archives of microbiology · 2022Article
- An Appraisal of Bacteriophage Isolation Techniques from Environment.Microbial ecology · 2022Review
- Genetic and phenotypic characteristics of Clostridium (Clostridioides) difficile from canine, bovine, and pediatric populations.Anaerobe · 2022Article
- Phage therapy forFrontiers in immunology · 2022Review
- Metagenomic-based characterization of the gut virome in patients with polycystic ovary syndrome.Frontiers in microbiology · 2022Article
- Clostridioides difficile phage biology and application.FEMS microbiology reviews · 2021Review
- Complete genome sequence of the newly discovered temperate Clostridioides difficile bacteriophage phiCDKH01 of the family Siphoviridae.Archives of virology · 2021Article
- Diversity and characterization of temperate bacteriophages induced in Pasteurella multocida from different host species.BMC microbiology · 2021Article
- First Comparative Analysis ofFrontiers in microbiology · 2021Article
- Integrative Longitudinal Analysis of Metabolic Phenotype and Microbiota Changes During the Development of Obesity.Frontiers in cellular and infection microbiology · 2021Article
- Bacteriophage endolysins as a potential weapon to combatGut microbes · 2020Review
- Phages in Anaerobic Systems.Viruses · 2020Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clostridium difficile is a Gram-positive pathogen infecting humans and animals. Recent studies suggest that animals could represent potential reservoirs of C. difficile that could then transfer to humans. Temperate phages contribute to the evolution of most bacteria, for example, by promoting the transduction of virulence, fitness, and antibiotic resistance genes. In C. difficile, little is known about their role, mainly because suitable propagating hosts and conditions are lacking. Here we report the isolation, propagation, and preliminary characterization of nine temperate phages from animal and human C. difficile isolates. Prophages were induced by UV light from 58 C. difficile isolates of animal and human origins. Using soft agar overlays with 27 different C. difficile test strains, we isolated and further propagated nine temperate phages: two from horse isolates (ΦCD481-1 and ΦCD481-2), three from dog isolates (ΦCD505, ΦCD506, and ΦCD508), and four from human isolates (ΦCD24-2, ΦCD111, ΦCD146, and ΦCD526). Two phages are members of the Siphoviridae family (ΦCD111 and ΦCD146), while the others are Myoviridae phages. Pulsed-field gel electrophoresis and restriction enzyme analyses showed that all of the phages had unique double-stranded DNA genomes of 30 to 60 kb. Phages induced from human C. difficile isolates, especially the members of the Siphoviridae family, had a broader host range than phages from animal C. difficile isolates. Nevertheless, most of the phages could infect both human and animal strains. Phage transduction of antibiotic resistance was recently reported in C. difficile. Our findings therefore call for further investigation of the potential risk of transduction between animal and human C. difficile isolates.
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