ArticleApplied and environmental microbiology2014
Conversion of 4-hydroxybutyrate to acetyl coenzyme A and its anapleurosis in the Metallosphaera sedula 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 30 citations in OpenAlex.
- An Oxygen-Tolerant Archaeal Dehydratase Enables a Synthetic Pathway for 4-Hydroxybutyrate-Containing Polyesters from Glucose and COACS synthetic biology · 2026Article
- Obligate heterotrophy of hyperthermophilic archaeamicroLife · 2026Article
- Development of a defined medium for the heterotrophic cultivation of Metallosphaera sedula.Extremophiles : life under extreme conditions · 2024Article
- Mechanistic constraints on the trade-off between photosynthesis and respiration in response to warming.Science advances · 2023Article
- Design and Construction of Artificial Biological Systems for One-Carbon Utilization.Biodesign research · 2023Review
- Convergent Evolution of a Promiscuous 3-Hydroxypropionyl-CoA Dehydratase/Crotonyl-CoA Hydratase inmSphere · 2021Article
- (Frontiers in microbiology · 2021Article
- Enzymes Catalyzing Crotonyl-CoA Conversion to Acetoacetyl-CoA During the Autotrophic COFrontiers in microbiology · 2020Article
- Global Transcriptional Programs in Archaea Share Features with the Eukaryotic Environmental Stress Response.Journal of molecular biology · 2019Review
- Structural insights into the inhibition properties of archaeon citrate synthase from Metallosphaera sedula.PloS one · 2019Article
- Reaction kinetic analysis of the 3-hydroxypropionate/4-hydroxybutyrate COMetabolic engineering · 2016Article
- Evaluation of 3-hydroxypropionate biosynthesis in vitro by partial introduction of the 3-hydroxypropionate/4-hydroxybutyrate cycle from Metallosphaera sedula.Journal of industrial microbiology & biotechnology · 2016Article
- Bioprocessing analysis of Pyrococcus furiosus strains engineered for CO₂-based 3-hydroxypropionate production.Biotechnology and bioengineering · 2015Article
- Novel Transcriptional Regulons for Autotrophic Cycle Genes in Crenarchaeota.Journal of bacteriology · 2015Article
- Malonic semialdehyde reductase from the archaeon Nitrosopumilus maritimus is involved in the autotrophic 3-hydroxypropionate/4-hydroxybutyrate cycle.Applied and environmental microbiology · 2015Article
- Ammonia-oxidizing archaea use the most energy-efficient aerobic pathway for CO2 fixation.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
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3 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The extremely thermoacidophilic archaeon Metallosphaera sedula (optimum growth temperature, 73°C, pH 2.0) grows chemolithoautotrophically on metal sulfides or molecular hydrogen by employing the 3-hydroxypropionate/4-hydroxybutyrate (3HP/4HB) carbon fixation cycle. This cycle adds two CO2 molecules to acetyl coenzyme A (acetyl-CoA) to generate 4HB, which is then rearranged and cleaved to form two acetyl-CoA molecules. Previous metabolic flux analysis showed that two-thirds of central carbon precursor molecules are derived from succinyl-CoA, which is oxidized to malate and oxaloacetate. The remaining one-third is apparently derived from acetyl-CoA. As such, the steps beyond succinyl-CoA are essential for completing the carbon fixation cycle and for anapleurosis of acetyl-CoA. Here, the final four enzymes of the 3HP/4HB cycle, 4-hydroxybutyrate-CoA ligase (AMP forming) (Msed_0406), 4-hydroxybutyryl-CoA dehydratase (Msed_1321), crotonyl-CoA hydratase/(S)-3-hydroxybutyryl-CoA dehydrogenase (Msed_0399), and acetoacetyl-CoA β-ketothiolase (Msed_0656), were produced recombinantly in Escherichia coli, combined in vitro, and shown to convert 4HB to acetyl-CoA. Metabolic pathways connecting CO2 fixation and central metabolism were examined using a gas-intensive bioreactor system in which M. sedula was grown under autotrophic (CO2-limited) and heterotrophic conditions. Transcriptomic analysis revealed the importance of the 3HP/4HB pathway in supplying acetyl-CoA to anabolic pathways generating intermediates in M. sedula metabolism. The results indicated that flux between the succinate and acetyl-CoA branches in the 3HP/4HB pathway is governed by 4-hydroxybutyrate-CoA ligase, possibly regulated posttranslationally by the protein acetyltransferase (Pat)/Sir2-dependent system. Taken together, this work confirms the final four steps of the 3HP/4HB pathway, thereby providing the framework for examining connections between CO2 fixation and central metabolism in M. sedula.
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